Volume 13, Number 3—March 2007
Clostridium difficile in Retail Ground Meat, Canada
|PCR ribotype*||PCR toxin genes†||Meat code||Ground beef product||Month sampled||Date sample processed||Store/brand code‡|
|077||A+B+, cdtB–, classic tcdC, toxinotype 0||M01||Regular||Jan||Jun 20||A/1|
|M26||A–B–, cdtB–, tcdC–, nontoxinotypeable||M26||Extra lean||May||Aug 10||A/1|
|M31||A+B+, cdtB+, type B/C tcdC, toxinotype III||M31||Regular patties||Aug||Sep 1||B/4|
|M47||Lean patties||Sep||Sep 6||B/4|
|M51||Lean patties||Sep||Sep 6||B/1|
|M52||Lean patties||Sep||Sep 6||B/4|
|014||A+B+, cdtB–, classic tcdC, toxinotype 0||M54§||Regular||Sep||Sep 20||D/2|
*077 and 014; nomenclature of Anaerobe Reference Laboratory, University of Wales, Cardiff, United Kingdom; M26 and M31 temporary nomenclature based on the PCR typing method of Bidet et al. (10). Eleven (91.6%) of 12 isolates were toxigenic.
†A, toxic gene tcdA; B, tcdB; cdtB, CDTb gene for the binding segment of the binary toxin; – and + indicate absence or presence of the gene. Classic tcdC, gene with no deletions; (≈345 bp); type B/C tcdC, gene with an ≈18-bp deletion (4).
‡Type of processed/packaged product; code 4, ground beef hamburgers sold as a special offer; other codes represent typical commercial ground beef packages.
§Commercial package from Quebec.