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Volume 13, Number 4—April 2007

Research

Flinders Island Spotted Fever Rickettsioses Caused by “marmionii” Strain of Rickettsia honei, Eastern Australia

Nathan B. Unsworth*, John Stenos*Comments to Author , Stephen R. Graves*, Antony G. Faa†, G. Erika Cox‡, John R. Dyer§, Craig S. Boutlis¶, Amanda M. Lane#, Matthew D. Shaw#, Jennifer Robson**, and Michael D. Nissen††
Author affiliations: *Australian Rickettsial Reference Laboratory, Geelong, Victoria, Australia; †Warwick Hospital (Southern Downs Health Services District), Warwick, Queensland, Australia; ‡Launceston General Hospital, Launceston, Tasmania, Australia; §Fremantle Hospital, Fremantle, Western Australia, Australia; ¶Menzies School of Health Research, Darwin, Northern Territory, Australia; #University of Queensland, Brisbane, Queensland, Australia; **Sullivan Nicolaides Pathology, Brisbane, Queensland, Australia; ††Royal Brisbane Hospital, Brisbane, Queensland, Australia

Main Article

Table 2

Oligonucleotide primers used for PCR amplification and sequencing

Primer Nucleotide sequence (5′→3′) Gene Reference
CS-F
TCG CAA ATG TTC ACG GTA CTT T
gltA
29
CS-R
TCG TGC ATT TCT TTC CAT TGT G
gltA
29
CS-P*
TGC AAT AGC AAG AAC CGT AGG CTG GAT G
gltA
29
MTO-1
GCT CTT GCA ACT CTA TGT T
orf17
12
MTO-2
CAT TGT TCG TCA GGT TGG CG
orf17
12
CS-162-F
GCA AGT ATC GGT GAG GAT GTA ATC
gltA
15
CS-398-SF
5′ATT ATG CTT GCG GCT GTC GG
gltA
15
CS-731-SR
AAG CAA AAG GGT TAG CTC C
gltA
15
RpCS1258
ATT GCA AAA AGT ACA GTG AAC A
gltA
17
rRNA1
AGA GTT TGA TCC TGG CTC AG
rrs
16
rRNA2
AAG GAG GTG ATC CAG CCG CA
rrs
16
rRNA3
CCC TCA ATT CCT TTG AGT TT
rrs
16
rRNA4
CAG CAG CCG CGG TAA TAC
rrs
16
Rr190.70p
ATG GCG AAT ATT TCT CCA AAA
ompA
17
Rr190.602n
AGT GCA GCA TTC GCT CCC CCT
ompA
17
D1f
ATG AGT AAA GAC GGT AAC CT
sca4
18
D928r
AAG CTA TTG CGT CAT CTC CG
sca4
18
D767f
CGA TGG TAG CAT TAA AAG CT
sca4
18
D1390r
CTT GCT TTT CAG CAA TAT CAC
sca4
18
D1219f
CCA AAT CTT CTT AAT ACA GC
sca4
18
D1876r
TAG TTT GTT CTG CCA TAA TC
sca4
18
D1738f
GTA TCT GAA TTA AGC AAT GCG
sca4
18
D2482r
CTA TAA CAG GAT TAA CAG CG
sca4
18
D2338f
GAT GCA GCG AGT GAG GCA GC
sca4
18
D3069r TCA GCG TTG TGG AGG GGA AG sca4 18

*5′ end labeled with 6-FAM; 3′ end labeled with BHQ-1.

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