Emerging Infectious Disease ISSN: 1080-6059

Chikungunya Virus, Cameroon, 2006

Christophe N. Peyrefitte*, Dominique Rousset†, Boris A.M. Pastorino*, Regis Pouillot†, Maël Bessaud*, Fabienne Tock*, Helene Mansaray‡, Olivier L. Merle*, Aurelie M. Pascual*, Christophe Paupy§, Aurelia Vessiere†, Patrice Imbert¶, Patrice Tchendjou†, Jean-Paul Durand*, Hugues J. Tolou*, and Marc Grandadam*

Author affiliations: *Institut de Médecine Tropicale du Service de Santé des Armées, Marseille, France; †Centre Pasteur du Cameroun, Yaoundé, Cameroon; ‡Centre Médico Social, Yaoundé, Cameroon; §Hôpital d'Instruction des Armées Begin, Clamart, France; ¶Institut de Recherche pour le Développement, Yaoundé, Cameroon;

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Figure

Phylogenetic tree of chikungunya virus (CHIKV) based on partial nucleotide sequences (3′ extremity of E1/3′-UTR, position 10,238–11,367). Phylogram was constructed with MEGA 2 program and tree drawing used the Jukes-Cantor algorithm for genetic distance determination and the neighbor-joining method. The percentage of successful bootstrap replicates (1,000 bootstrap replications, confidence probability >90%) is indicated at the nodes. The length of branches is proportional to the number of nucleotide changes (% of divergence). Asterisk (*) and arrow indicate the strains isolated in this work. The dark triangle corresponds to viruses clustering together. O’nyong-nyong virus (ONNV) sequence has been introduced for correct rooting of the tree. The GenBank reference no. for the Cameroon CHIKV isolate is EF051584.

Figure. Phylogenetic tree of chikungunya virus (CHIKV) based on partial nucleotide sequences (3′ extremity of E1/3′-UTR, position 10,238–11,367). Phylogram was constructed with MEGA 2 program and tree drawing used the Jukes-Cantor algorithm for genetic distance determination and the neighbor-joining method. The percentage of successful bootstrap replicates (1,000 bootstrap replications, confidence probability >90%) is indicated at the nodes. The length of branches is proportional to the number of nucleotide changes (% of divergence). Asterisk (*) and arrow indicate the strains isolated in this work. The dark triangle corresponds to viruses clustering together. O’nyong-nyong virus (ONNV) sequence has been introduced for correct rooting of the tree. The GenBank reference no. for the Cameroon CHIKV isolate is EF051584.

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