Christophe N. Peyrefitte*, Dominique Rousset†, Boris A.M. Pastorino*, Regis Pouillot†, Maël Bessaud*, Fabienne Tock*, Helene Mansaray‡, Olivier L. Merle*, Aurelie M. Pascual*, Christophe Paupy§, Aurelia Vessiere†, Patrice Imbert¶, Patrice Tchendjou†, Jean-Paul Durand*, Hugues J. Tolou*, and Marc Grandadam*
Author affiliations: *Institut de Médecine Tropicale du Service de Santé des Armées, Marseille, France; †Centre Pasteur du Cameroun, Yaoundé, Cameroon; ‡Centre Médico Social, Yaoundé, Cameroon; §Hôpital d'Instruction des Armées Begin, Clamart, France; ¶Institut de Recherche pour le Développement, Yaoundé, Cameroon;
Figure. Phylogenetic tree of chikungunya virus (CHIKV) based on partial nucleotide sequences (3′ extremity of E1/3′-UTR, position 10,238–11,367). Phylogram was constructed with MEGA 2 program and tree drawing used the Jukes-Cantor algorithm for genetic distance determination and the neighbor-joining method. The percentage of successful bootstrap replicates (1,000 bootstrap replications, confidence probability >90%) is indicated at the nodes. The length of branches is proportional to the number of nucleotide changes (% of divergence). Asterisk (*) and arrow indicate the strains isolated in this work. The dark triangle corresponds to viruses clustering together. O’nyong-nyong virus (ONNV) sequence has been introduced for correct rooting of the tree. The GenBank reference no. for the Cameroon CHIKV isolate is EF051584.
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