Afia Zafar* , Robert Swanepoel†, Roger Hewson‡, Mazhar Nizam§, Altaf Ahmed§, Akhtar Husain*, Antoinette Grobbelaar†, Kevin Bewley‡, Valerie Mioulet‡, Barry Dowsett‡, Linda Easterbrook‡, and Rumina Hasan*
Author affiliations: *Aga Khan University Hospital, Karachi, Pakistan; †National Institute for Communicable Diseases, Sandringham, South Africa; ‡Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK; §Patel Hospital, Karachi, Pakistan;
Figure 2. Maximum likelihood phylogenetic tree based on a 955-nt alignment of the Karachi isolate and 33 orthopoxvirus sequences of the B5R gene from GenBank constructed with ClustalW (www.ebi.ac.uk/clustalw/index.html) and TREE-PUZZLE (http://bioweb.pasteur.fr/seqanal/interfaces/puzzle.html); figures at nodes represent PUZZLE support values. The orthopoxvirus types are indicated to the right. The Karachi isolate sequence (Pakistan 2005) groups within the buffalopox B5R genes.
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