Emerging Infectious Disease ISSN: 1080-6059

Nosocomial Buffalopoxvirus Infection, Karachi, Pakistan

Afia Zafar*

, Robert Swanepoel†, Roger Hewson‡, Mazhar Nizam§, Altaf Ahmed§, Akhtar Husain*, Antoinette Grobbelaar†, Kevin Bewley‡, Valerie Mioulet‡, Barry Dowsett‡, Linda Easterbrook‡, and Rumina Hasan*

Author affiliations: *Aga Khan University Hospital, Karachi, Pakistan; †National Institute for Communicable Diseases, Sandringham, South Africa; ‡Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK; §Patel Hospital, Karachi, Pakistan;

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Figure 2

Maximum likelihood phylogenetic tree based on a 955-nt alignment of the Karachi isolate and 33 orthopoxvirus sequences of the B5R gene from GenBank constructed with ClustalW (www.ebi.ac.uk/clustalw/index.html) and TREE-PUZZLE (http://bioweb.pasteur.fr/seqanal/interfaces/puzzle.html); figures at nodes represent PUZZLE support values. The orthopoxvirus types are indicated to the right. The Karachi isolate sequence (Pakistan 2005) groups within the buffalopox B5R genes.

Figure 2. Maximum likelihood phylogenetic tree based on a 955-nt alignment of the Karachi isolate and 33 orthopoxvirus sequences of the B5R gene from GenBank constructed with ClustalW (www.ebi.ac.uk/clustalw/index.html) and TREE-PUZZLE (http://bioweb.pasteur.fr/seqanal/interfaces/puzzle.html); figures at nodes represent PUZZLE support values. The orthopoxvirus types are indicated to the right. The Karachi isolate sequence (Pakistan 2005) groups within the buffalopox B5R genes.

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