Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
Shanthi Jayawardena*, Chung Y. Cheung*, Ian G. Barr†, Kwok H. Chan*, Honglin Chen*, Yi Guan*, J.S. Malik Peiris*, and Leo L.M. Poon*
Author affiliations: *The University of Hong Kong, Hong Kong Special Administrative Region, People’s Republic of China; †World Health Organization Collaborating Centre for Influenza, Melbourne, Victoria, Australia;
Appendix Figure 1. Visual inspection of the positivity of LAMPreactions. Heated, treated viral culture was serially diluted and tested by the LAMP assay. Reactions were visually inspected after the incubation. Positive reactions would produce large amounts of white magnesium pyrophosphate precipitate, thereby increasing the turbidity of these reactions (+ve control and samples 1 to 3). By contrast, negative reactions (-ve control and sample 4) remained transparent after the incubation. The amount of pfu used in the tested samples is indicated.
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