Emerging Infectious Disease ISSN: 1080-6059

Ilheus Virus Isolate from a Human, Ecuador

Barbara W. Johnson*

, Cristopher Cruz†, Vidal Felices†, William R. Espinoza‡, Stephen Robert Manock§, Carolina Guevara†, James G. Olson†, and Tadeusz J. Kochel†

Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA; †US Naval Medical Research Center Detachment, Lima, Peru; ‡Hospital de la IV División del Ejercito “Amazonas,” Puyo, Ecuador; §Hospital Vozandes del Oriente, Shell, Ecuador;

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Figure

Phylogenetic analysis of the NS5 (A) and E gene (B) regions of 6 Ilheus virus (ILHV) isolates. The sequences were aligned in MegAlign (DNASTAR, Inc., Madison, WI, USA); the alignments were then analyzed by using the maximum parsimony method with 500 bootstrap replicates in the program MEGA 3.1 (10). Rocio virus (GenBank accession no. AY632542) was included as an outgroup in the analysis, based on the phylogram of Kuno and Chang (1). Bootstrap values, shown in boldface above the branch, are percentages derived from 500 samplings; branch lengths are shown below the branch. The sequences generated from our study were deposited in GenBank under accession nos. EF396941–EF396952.

Figure. Phylogenetic analysis of the NS5 (A) and E gene (B) regions of 6 Ilheus virus (ILHV) isolates. The sequences were aligned in MegAlign (DNASTAR, Inc., Madison, WI, USA); the alignments were then analyzed by using the maximum parsimony method with 500 bootstrap replicates in the program MEGA 3.1 (10). Rocio virus (GenBank accession no. AY632542) was included as an outgroup in the analysis, based on the phylogram of Kuno and Chang (1). Bootstrap values, shown in boldface above the branch, are percentages derived from 500 samplings; branch lengths are shown below the branch. The sequences generated from our study were deposited in GenBank under accession nos. EF396941–EF396952.

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