Volume 13, Number 8—August 2007
Dispatch
Babesia sp. EU1 from Roe Deer and Transmission within Ixodes ricinus
Sarah Bonnet*
, Maggy Jouglin*, Monique L’Hostis†, and Alain Chauvin†
, Maggy Jouglin*, Monique L’Hostis†, and Alain Chauvin†Table 1
Nucleotide sequences of PCR primers used for amplification and sequencing of 18S rRNA genes of Babesia spp.*
| Primer | Specificity | Sequence (5′→3′) | Annealing temperature, °C | Fragment size, bp | Reference |
|---|---|---|---|---|---|
| BAB | Babesia/Theileria spp. | 60 | 359 | (11) | |
| GF2 | GYYTTGTAATTGGAATGATGG | ||||
| GR2 | CCAAAGACTTTGATTTCTCTC | ||||
| EU1 | Babesia sp. EU1 | 63 | 362 | (8) | |
| Up | GTTTCTGMCCCATCAGCTTGAC | ||||
| Down | AGACAAGAGTCAATAACTCGATAAC | ||||
| CRYPTO | Apicomplexa | 65 | 1,727 | (4) | |
| F | AACCTGGTTGATCCTGCCAGTAGTCAT | ||||
| R | GAATGATCCTTCCGCAGGTTCACCTAC |
*For parasites from tick samples, no sequence could be obtained with primer set CRYPTO because such primers likely hybridize to the Ixodes ricinus 18S rRNA gene and preferentially amplified this gene, probably because of its relative abundance.
