Classic Scrapie in Sheep with the ARR/ARR Prion Genotype in Germany and France
Martin H. Groschup*1 , Caroline Lacroux†1, Anne Buschmann*, Gesine Lühken‡, Jacinthe Mathey†, Martin Eiden*, Séverine Lugan†, Christine Hoffmann*, Juan Carlos Espinosa§, Thierry G.M. Baron¶, Juan Maria Torres§, Georg Erhardt‡, and Olivier Andreoletti†
Author affiliations: *Friedrich-Loeffler-Institut, Insel Riems, Germany; †Institut National de la Recherche Agronimique, Toulouse, France; ‡Justus-Liebig–University Giessen, Giessen, Germany; §Centro de Investigación en Sanidad Animal, Madrid, Spain; ¶Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;
Figure 1. Antibody-binding patterns of the prion protein (PrPSc) associated with cases of ARR/ARR scrapie in France and Germany. A) and B) Western blots showing the differences in monoclonal antibody (MAb) P4 binding compared with the internal standard MAb L42 of PrPSc derived from S115/04 (ARR/ARR Germany), S83 (ARR/ARR France), ovine ARQ/ARQ bovine spongiform encephalopathy (BSE), and S95 (classic scrapie) cases. Banding intensities were quantified by photoimaging, and binding ratios were calculated. Note the significantly weaker P4 binding to the ovine BSE sample. Lane 1, S115/04; lane 2, S83; lane 3, S95; lane 4, ovine BSE; lane 5, atypical S15. C) Relative MAb binding ratios for lane nos. 1–4 in the Western blots shown in A) and B).
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