Classic Scrapie in Sheep with the ARR/ARR Prion Genotype in Germany and France
Martin H. Groschup*1 , Caroline Lacroux†1, Anne Buschmann*, Gesine Lühken‡, Jacinthe Mathey†, Martin Eiden*, Séverine Lugan†, Christine Hoffmann*, Juan Carlos Espinosa§, Thierry G.M. Baron¶, Juan Maria Torres§, Georg Erhardt‡, and Olivier Andreoletti†
Author affiliations: *Friedrich-Loeffler-Institut, Insel Riems, Germany; †Institut National de la Recherche Agronimique, Toulouse, France; ‡Justus-Liebig–University Giessen, Giessen, Germany; §Centro de Investigación en Sanidad Animal, Madrid, Spain; ¶Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;
Figure 3. Lesion profiling (A, B, C) and paraffin-embedded tissue blot characterization of prion protein (PrPSc) deposition at thalamic level (D, E, F). Tests were performed by using formalin-fixed brain from Tg338 mice (expressing the VRQ PrP ovine variant) inoculated with (A, D) ARR/ARR atypical case (B, E) bovine spongiform encephalopathy (BSE) brain from an ARR/ARR sheep (intracerebral inoculation), and (C, F) case S83. Each lesion profile was carried out by using 6 animals. Detection of PrPSc was achieved by using the monoclonal antibody Sha31.
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