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Volume 14, Number 10—October 2008

Letter

Detecting Avian Influenza Virus (H5N1) in Domestic Duck Feathers

Yu YamamotoComments to Author , Kikuyasu Nakamura, Masatoshi Okamatsu, Ayako Miyazaki, Manabu Yamada, and Masaji Mase
Author affiliations: National Institute of Animal Health, Tsukuba, Japan;

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Appendix Figure

A) Developing contour feather. The calamus was used for examination (bar = 1 cm). B) Result of the rapid test with feathers. A pink line (arrowhead) indicates a positive result for influenza A virus. C) Immunohistochemical stain of a biopsied feather composed of feather epidermis (fe) and feather pulp (fp). Influenza virus nucleoprotein was detected in the fe epidermal cells (arrowheads) (bar = 200  m).

Appendix Figure. A) Developing contour feather. The calamus was used for examination (bar = 1 cm). B) Result of the rapid test with feathers. A pink line (arrowhead) indicates a positive result for influenza A virus. C) Immunohistochemical stain of a biopsied feather composed of feather epidermis (fe) and feather pulp (fp). Influenza virus nucleoprotein was detected in the fe epidermal cells (arrowheads) (bar = 200 m).

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