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Volume 14, Number 2—February 2008

Research

Unexpected Occurrence of Plasmid-Mediated Quinolone Resistance Determinants in Environmental Aeromonas spp.

Vincent Cattoir*†‡, Laurent Poirel*†, Camille Aubert*†, Claude-James Soussy‡, and Patrice Nordmann*†Comments to Author 
Author affiliations: *Institut Nationale de la Santé et de la Recherche Médicale Unité 914, Le Kremlin-Bicêtre, France; †Hôpital de Bicêtre, Le Kremlin-Bicêtre; ‡Université Paris XII, Créteil, France;

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Figure 2

Genetic environments of the qnrS2 gene in plasmid p37 from Aeromonas punctata 37 and comparison with related plasmid structures. Plasmid pFBAOT6 is from A. punctata from the United Kingdom (23); plasmids pGNB2 and pMG308 are from a wastewater treatment plant from Germany (unknown bacterial reservoir) (24) and from a non-Typhi Salmonella clinical isolate from the United States (25), respectively. Recombinant plasmid pAS37 has been obtained from our study. Open reading frames (ORFs) are indicated by horizontal arrows. The right and left inverted repeats (IRR and IRL) are indicated, and duplication sites (CCTCC) are represented by black triangles. The EcoRI- restriction sites that have been used for cloning experiments are indicated. The identified mobile insertion cassette element is bracketed by IRL and IRR of 22-bp size (bases in black are identical, and bases in white are different).

Figure 2. Genetic environments of the qnrS2 gene in plasmid p37 from Aeromonas punctata 37 and comparison with related plasmid structures. Plasmid pFBAOT6 is from A. punctata from the United Kingdom (23); plasmids pGNB2 and pMG308 are from a wastewater treatment plant from Germany (unknown bacterial reservoir) (24) and from a non-Typhi Salmonella clinical isolate from the United States (25), respectively. Recombinant plasmid pAS37 has been obtained from our study. Open reading frames (ORFs) are indicated by horizontal arrows. The right and left inverted repeats (IRR and IRL) are indicated, and duplication sites (CCTCC) are represented by black triangles. The EcoRI- restriction sites that have been used for cloning experiments are indicated. The identified mobile insertion cassette element is bracketed by IRL and IRR of 22-bp size (bases in black are identical, and bases in white are different).

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