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Volume 14, Number 2—February 2008

Research

Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum

Laurence Delhaes*12, Azian Harun*†1, Sharon C.A. Chen*1, Quoc Nguyen‡1, Monica Slavin§, Christopher H. Heath¶#, Krystyna Maszewska*†, Catriona Halliday*, Vincent Robert**, Tania C. Sorrell*, Wieland Meyer*†1Comments to Author , and the Australian Scedosporium (AUSCEDO) Study Group
Author affiliations: *Westmead Hospital, Westmead, New South Wales, Australia; †University of Sydney, Sydney, New South Wales, Australia; ‡St. Vincent’s Hospital, Sydney, New South Wales, Australia; §Alfred Hospital, Melbourne, Victoria, Australia; ¶Royal Perth Hospital, Perth, Western Australia, Australia; #University of Western Australia, Perth, Western Australia, Australia; **Centraalbureau voor Schimmelcultures, Utrecht, the Netherlands;

Main Article

Table 1

Primer and adaptor oligonucleotide sequences used in the study

Primer or adaptor oligonucelotideSequence (5′→3′)
rDNA primers
SR6RAAGTARAAGTCGTAACAAGG
LR1
GGTTGGTTTCTTTTCCT
M13 fingerprinting primer
Phage M13
GAGGGTGGCGGTTCT
EcoRI adapters
EA1CTCGTAGACTGCGTACC
EA2
CATCTGACGCATGGTTAA
Msel adapters
MA1GACGATGAGTCCTGAG
MA2
TACTCAGGACTCAT
Preselective primers
EcoRI-TGACTGCGTACCAATTCT
EcoRI-GGACTGCGTACCAATTCG
Msel-CGATGAGTCCTGAGTAAC
Msel-G
GATGAGTCCTGAGTAAG
Selective primers
EcoRI-TG6 FAM-GACTGCGTACCAATTCTG
EcoRI-GT6 FAM-GACTGCGTACCAATTCGT
Msel-CAGATGAGTCCTGAGTAACA
Msel-GTGATGAGTCCTGAGTAAGT

Main Article

1These authors contributed equally to experimental work and data analysis.

2Current affiilation: Lille Pasteur Institute, Lille, France

3Members of the Australian Scedosporium Study Group of the Australasian Society for Infectious Diseases: Australian Capital Territory: Peter Collignon; The Canberra Hospital. New South Wales: Richard Benn (Royal Prince Alfred Hospital); Ian Chambers (Douglass Hanly Moir Pathology); Sharon Chen (Westmead Hospital); Nelson Dennis (Wollongong Hospital); Deo DeWit (Gosford Hospital); John Ferguson (John Hunter Hospital); Iain Gosbell (Liverpool Hospital); Thomas Gottlieb (Concord Hospital); Catriona Halliday (Westmead Hospital); Juliette Holland (Mayne Laverty Pathology); Alison Kesson (New Children’s Hospital, Westmead); Richard Lawrence (St. George Hospital); Deborah Marriott (St. Vincent’s Hospital, Sydney); Wieland Meyer (Westmead Hospital); Peter Newton (Wollongong Hospital); Quoc Nguyen (St. Vincent’s Hospital, Sydney); Pamela Palasanthrian (Sydney Children’s Hospital); Robert Pickles (infectious diseases physician, Taree), Robert Pritchard (Royal North Shore Hospital); Tania Sorrell (Westmead Hospital); Lex Tierney (John Hunter Hospital); Voula Tomasotos (Liverpool Hospital); Robert Vaz (Orange Base Hospital); Kerry Weeks (Royal North Shore Hospital). Queensland: Anthony Allworth (Royal Brisbane Hospital); Christopher Coulter (The Prince Charles Hospital); Joan Faoagali (Royal Brisbane Hospital); Barbara Johnson (Princess Alexandra Hospital), David Looke (Princess Alexandra Hospital), Joseph McCormack (The Mater Adult Hospital); Graeme Nimmo (Princess Alexandra Hospital); Gabrielle O’Kane (The Prince Charles Hospital); E. Geoffrey Playford (Princess Alexandra Hospital); Jennifer Robson (Sullivan and Nicolaides Pathology). South Australia: David Ellis (Women’s and Children’s Hospital); Rosemary Handke (Women’s and Children’s Hospital); Karen Rowlands (Royal Adelaide Hospital); David Shaw(Royal Adelaide Hospital). Tasmania: Louise Cooley (Royal Hobart Hospital); Erica Cox (Launceston General Hospital); Alistair McGregor (Royal Hobart Hospital). Victoria: Clare Franklin (Alfred Hospital); Cathy Joseph (St Vincent’s Hospital, Melbourne), Tony Korman (Monash Medical Centre), Orla Morrissey (Alfred Hospital), Monica Slavin (Peter MacCallum Cancer Centre), Denis Spelman (Alfred Hospital); Bryan Speed (Austin and Repatriation Hospitals); Harsha Sheorey (St. Vincent’s Hospital, Melbourne). Western Australia: Peter Boan (Royal Perth Hospital); John Dyer (Fremantle Hospital); Christopher Heath (Royal Perth Hospital); Dianne Gardam (Royal Perth Hospital); Duncan McLennan (Fremantle Hospital); Ronan Murray (Royal Perth Hospital); Todd Pryce (Royal Perth Hospital).

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