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Volume 14, Number 7—July 2008

Letter

Klebsiella pneumoniae Carbapenemase–2, Buenos Aires, Argentina

Fernando G. Pasteran*Comments to Author , Luis Otaegui*, Leonor Guerriero*, Gabriel Radice†, Ricardo Maggiora†, Melina Rapoport*, Diego Faccone*, Ana Di Martino†, and Marcelo Galas*
Author affiliations: *Instituto Nacional de Enfermedades Infecciosas–ANLIS “Dr. Carlos G. Malbran,” Ciudad Autónoma de Buenos Aires, Argentina; †Sanatorio Trinidad Mitre, Ciudad Autónoma de Buenos Aires;

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Table

Antibiotic susceptibility, isolectric focusing of β-lactamases and PCR of antimicrobial resistance determinants in Klebsiella pneumoniae, Citrobacter freundii clinical isolates, Salmonella transconjugants, and recipient and control strains*

Type of testing Clinical isolates
Transconjugants and recipient strains
Control strains
K.p. M9171 C.f. M9169 Salmonella
M9204† Salmonella
M9190‡ Salmonella M1744 K.p. D5/07§ K.p. C2¶ E. coli ATCC 25922
MICs (µg/mL)
Antimicrobial agent
Imipenem 32 1.0 1.0 1.0 0.12 2.0 0.12 0.25
Impenem/clavulanate 32 0.25 0.25 0.25 0.12 0.5 0.12 0.25
Impenem/APB 2.0 0.12 0.25 0.12 0.12 0.12 0.12 0.25
Imipenem/EDTA# 32 0.25 1.0 1.0 0.12 2.0 0.12 0.12
Meropenem 32 1.0 0.5 1.0 0.015 2.0 0.03 0.03
Meropenem/clavulanate 32 0.12 0.03 0.06 0.015 1.0 0.03 0.03
Meropenem/APB 1.0 0.06 0.03 0.03 0.015 0.12 0.03 0.03
Meropenem/EDTA# 32 1.0 0.5 1.0 0.015 2.0 0.03 0.03
Ertapenem 128 2.0 1.0 1.0 0.008 16 0.06 0.015
Ertapenem/clavulanate 128 0.25 0.03 0.06 0.008 16 0.06 0.008
Ertapenem/APB 8.0 0.008 0.015 0.03 0.008 0.5 0.03 0.008
Cefoxitin 64 64 4.0 8.0 2.0 ND ND 4
Ceftazidime 256 16 16 32 0.06 ND ND 0.25
Ceftazidime/clavulante 16 32 0.5 0.5 0.06 ND ND ND
Cefepime 32 4.0 16 16 0.03 ND ND 0.03
Cefepime/clavulanate 16 0.25 0.5 0.25 0.03 ND ND ND
Tigecycline**
1.0
0.25

ND
ND
ND

ND
ND
0.25
Isolectric focusing results
pI band††
5.4 + 6.7 + 7.6
5.4 + 6.7 + >9.0

6.7
6.7
None

ND
ND
None
PCR results
Β-lactamase
blaKPC + + + + + ND
blaampC + 
CIT/
CMY +
CIT/
CMY ND
blaPER-2 + ND ND ND
blaSHV + ND ND ND
blaTEM-1 + + ND ND ND

*K.p., Klebsiella pneumoniae; C.f., Citrobacter freundii; E. coli, Escherichia coli; APB, 3-aminophenylboronic acid; IEF, isoelectric focusing; pI, isoelectric point; ND, not determined; +, positive; –, negative.
†M9204 transconjugant derived from K. pneumoniae M9171.
‡M9190 transconjugant derived from C. freundii M9169.
§Control strain producing KPC-2.
¶Control strain producing CMY-2.
#EDTA 0.4 mmol/L.
**Microdilution (JustOne, Trek Diagnostic Systems, Cleveland, OH, USA). Breakpoints according to US Food and Drug Administration (susceptible <2 μg/mL).
††Underlined pI bands indicated activity against third-generation cephalosporins

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