Volume 14, Number 7—July 2008
Research
Wide Distribution of a High-Virulence Borrelia burgdorferi Clone in Europe and North America
Wei-Gang Qiu*
, John F. Bruno†, William D. McCaig*, Yun Xu†, Ian Livey‡, Martin E. Schriefer§, and Benjamin J. Luft†
, John F. Bruno†, William D. McCaig*, Yun Xu†, Ian Livey‡, Martin E. Schriefer§, and Benjamin J. Luft†Table 2
Genomic markers and PCR primers
| Locus* | Primer sequence (5′ → 3′)† | Location‡ |
|---|---|---|
| BB0057 (gap) | F-ATGAAATTGGCTATTAATGG, R-TTGAGCAAGATCAACCACTC | Main chromosome (52.5 K) |
| BB0160 (alr) | F-ATGTATAATAATAAAACAATGG, R-ATTTTCTCTTTTCGTATTTTCC | Main chromosome (160 K) |
| BB0243 (glpA) | F-ATGGAGGAATATTTAAATTTC, R-GTTCATTTTTCCACTCTTC | Main chromosome (249 K) |
| IGS (rrs-rrlA) | 1st round§: F-GGTATGTTTAGTGAGGG, R-GGTTAGAGCGCAGGTCTG; 2nd round: F-CGTACTGGAAAGTGCGGCTG, R-GATGTTCAACTCATCCTGGTCCC | Main chromosome (444 K) |
| BB0545 (xylB) | F-ATGAATGCTCTTAGTATTG, R-CCCGTTAACAAATAGAC | Main chromosome (555 K) |
| BB0622 (ackA) | F-TTGTCAAATACAAAAGG, R-AATGTCTTCAAGAATGG | Main chromosome (649 K) |
| BB0809 (tgt) | F-ATGTTTAGTGTAATCAAGAATG, R-ATCGAAATTTTCCTCTTCATAC | Main chromosome (855 K) |
| BBA24 (dpbA) | F1-TAATGTTATGATTAAATG, F2-ATGAATAAATATCAAAAAAC, R-GAAATTCCAAATAACATC | lp54 |
| BBB19 (ospC) | F-CCGTTAGTCCAATGGCTCCAG, R-ATGCAAATTAAAGTTAATATC | cp26 |
| BBD14 | F-ATGATAATAAAAATAAAAAATAATG, R-ATTTTGATTAATTTTAATTTTGCTG | lp17 |
