Volume 14, Number 7—July 2008
Research
Wide Distribution of a High-Virulence Borrelia burgdorferi Clone in Europe and North America
Wei-Gang Qiu*
, John F. Bruno†, William D. McCaig*, Yun Xu†, Ian Livey‡, Martin E. Schriefer§, and Benjamin J. Luft†
, John F. Bruno†, William D. McCaig*, Yun Xu†, Ian Livey‡, Martin E. Schriefer§, and Benjamin J. Luft†Table 3
Analysis of molecular variance results*†
| Locus | Molecular variance, % |
Nucleotide diversity, π |
Fixation index (FST)‡ | |||
|---|---|---|---|---|---|---|
| Between continents | Within continents | North America | Europe | |||
| IGS | 19.5 | 80.5 | 0.0253 | 0.0243 | 0.1952§ | |
| ospC | 3.13 | 96.87 | 0.2066 | 0.1900 | 0.0313¶ | |
| dbpA | 26.5 | 73.5 | 0.1480 | 0.0999 | 0.2650§ | |
| BBD14 | 2.54 | 97.46 | 0.0834 | 0.1333 | 0.0254 (NS) | |
*IGS, intergenic spacer; ospC, outer surface protein C; NS, not significant (p>0.05).
†Results were obtained by using Arlequin 3.1 (35). Samples were 66 IGS sequences divided into 2 continental populations: North America (36 sequences from New York, Connecticut, Massachusetts, and Michigan) and Europe (30 sequences from Italy, Austria, France, Germany, Switzerland, Poland, Hungary, Slovenia, and Finland). Two outgroup sequences (SV1 and Ri5) were excluded from the European sample. Genetic distances between haplotypes were based on the Kimura 2-parameter model.
‡Levels of significance were obtained by 1,000 permutations.
§p<0.001.
¶0.01<p<0.05.
