Transmission of Bartonella henselae by Ixodes ricinus
Violaine Cotté*, Sarah Bonnet*, Danielle Le Rhun*, Evelyne Le Naour*, Alain Chauvin†, Henri-Jean Boulouis‡, Benoit Lecuelle‡, Thomas Lilin‡, and Muriel Vayssier-Taussat*
Author affiliations: *Institut National de la Recherche Agronomique, Maisons-Alfort, France; †École Nationale Vétérinaire de Nantes, Nantes, France; ‡École Nationale Vétérinaire d’Alfort, Maisons-Alfort, France;
Figure 2. Seminested PCR detection of Bartonella spp. DNA in Ixodes ricinus ticks fed on B. henselae–infected ovine blood at preceding stage. Lane M, 100-bp DNA molecular mass marker; lane Ags, salivary glands of a female adult fed on infected blood as a nymph; lane A, carcass of a female adult fed on infected blood as a nymph; lane Ngs, salivary glands of a nymph fed on infected blood as a larva; lane N, carcass of a nymph fed on infected blood as a larva; lane E, eggs laid by female adult fed on infected blood; lane L, larvae hatched from female adult fed on infected blood; lane T+, B. bacilliformis DNA; lane T–, nymph fed on uninfected ovine blood.
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