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Volume 14, Number 7—July 2008

Research

Transmission of Bartonella henselae by Ixodes ricinus

Violaine Cotté*, Sarah Bonnet*, Danielle Le Rhun*, Evelyne Le Naour*, Alain Chauvin†, Henri-Jean Boulouis‡, Benoit Lecuelle‡, Thomas Lilin‡, and Muriel Vayssier-Taussat*Comments to Author 
Author affiliations: *Institut National de la Recherche Agronomique, Maisons-Alfort, France; †École Nationale Vétérinaire de Nantes, Nantes, France; ‡École Nationale Vétérinaire d’Alfort, Maisons-Alfort, France;

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Figure 2

Seminested PCR detection of Bartonella spp. DNA in Ixodes ricinus ticks fed on B. henselae–infected ovine blood at preceding stage. Lane M, 100-bp DNA molecular mass marker; lane Ags, salivary glands of a female adult fed on infected blood as a nymph; lane A, carcass of a female adult fed on infected blood as a nymph; lane Ngs, salivary glands of a nymph fed on infected blood as a larva; lane N, carcass of a nymph fed on infected blood as a larva; lane E, eggs laid by female adult fed on infected blood; lane L, larvae hatched from female adult fed on infected blood; lane T+, B. bacilliformis DNA; lane T–, nymph fed on uninfected ovine blood.

Figure 2. Seminested PCR detection of Bartonella spp. DNA in Ixodes ricinus ticks fed on B. henselae–infected ovine blood at preceding stage. Lane M, 100-bp DNA molecular mass marker; lane Ags, salivary glands of a female adult fed on infected blood as a nymph; lane A, carcass of a female adult fed on infected blood as a nymph; lane Ngs, salivary glands of a nymph fed on infected blood as a larva; lane N, carcass of a nymph fed on infected blood as a larva; lane E, eggs laid by female adult fed on infected blood; lane L, larvae hatched from female adult fed on infected blood; lane T+, B. bacilliformis DNA; lane T–, nymph fed on uninfected ovine blood.

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