AIDS Patient Death Caused by Novel Cryptococcus neoformans × C. gattii Hybrid
Marjan Bovers*12, Ferry Hagen*†1 , Eiko E. Kuramae*, Hans L. Hoogveld‡, Françoise Dromer§, Guy St-Germain¶, and Teun Boekhout*†
Author affiliations: *Centraalbureau voor Schimmelcultures–Fungal Biodiversity Centre, Utrecht, the Netherlands; †University Medical Centre Utrecht, Utrecht; ‡Netherlands Institute of Ecology, Nieuwersluis, the Netherlands; §Institut Pasteur, Paris, France; ¶Laboratoire Santé Publique Quebec, Sainte-Anne-de-Bellevue, Quebec, Canada;
Figure 1. A) Determination of ploidy of the novel Cryptococcus neoformans × C. gattii serotype AB hybrid isolate CBS10496 by flow cytometry. The first peak corresponds to the G1 phase; the second peak corresponds to the G2 phase. Haploid reference strain CBS10510 is shown by the red line; CBS10496 is shown by the black line. The G1 peak of CBS10496 coincided with the G2 peak of strain CBS10510, which indicated that strain CBS10496 has approximately twice the amount of DNA than CBS10510. B) Nuclear staining of isolate CBS10496 with 4′,6-diamidino-2-phenylindole, showing that cells are monokaryotic. Scale bar = 10 μm.
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