Volume 14, Number 9—September 2008
Dispatch
Neurobrucellosis in Stranded Dolphins, Costa Rica
Gabriela Hernández-Mora, Rocío González-Barrientos, Juan-Alberto Morales, Esteban Chaves-Olarte, Caterina Guzmán-Verri, Elías Baquero-Calvo, María-Jesús De-Miguel, Clara-María Marín, José-María Blasco, and Edgardo Moreno
Table
Characterization of Brucella spp. isolated from Stenella coeruleoalba and comparison with marine reference strains
| Species, strain§ | CO2¶ | Serum against† |
Growth on dyes, μg/mL‡ |
Omp2b restriction pattern | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RTD phage lysis* |
Thionin |
Basic fuchsin |
O safranin |
||||||||||||||
| Tb | Wb | Iz | R/C | A | M | 10 | 20 | 40 | 100 | 20 | 100 | ||||||
| B. ceti, B14/94# | – | + | + | – | – | + | + | + (+) | + (±) | + (–) | + (+) | + (–) | + (+) | Dolphin type I | |||
| B. ceti, B1/94# | – | + | – | – | – | + | – | + (+) | + (+) | + (+) | + (–) | + (–) | + (–) | Porpoise type II | |||
| B. pinnipedialis, B2/94# | – | – | + | – | + | + | – | + (–) | + (–) | + (–) | + (–) | + (–) | + (–) | Seal | |||
| B. ceti, B1 to B6** | – | + | + | – | – | + | + | + (+) | + (–) | – (–) | + (–) | + (–) | + (–) | Dolphin type I | |||
*RTD, routine test dilution of phages Tbilisi (Tb), Weybridge (Wb), Izatnagar (Iz), and rough type Wb derivative (R/C).
†Serum against lipopolysaccharide epitopes, measured as agglutination with monospecific serum.
‡Dye concentrations expressed in µg/mL of culture medium with 10% CO2 or, within parenthesis, raw incubation without CO2.
§All reference and isolated strains were SH2 negative, urease positive and amplified Brucella spp L12 rRNA by PCR.
¶Requirement.
#Reference strains (11,12).
**Isolates from Costa Rica.
