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Volume 14, Number 9—September 2008

Research

Pediatric Parapneumonic Empyema, Spain

Ignacio ObandoComments to Author , Carmen Muñoz-Almagro, Luis A. Arroyo, David Tarrago, David Sanchez-Tatay, David Moreno-Perez, Sahar S. Dhillon, Cristina Esteva, Susanna Hernandez-Bou, Juan J. Garcia-Garcia, William P. Hausdorff, and Angela B. Brueggemann
Author affiliations: Virgen del Rocio Children’s Hospital, Seville, Spain (I. Obando, L.A. Arroyo, D. Sanchez-Tatay); Sant Joan de Deu Hospital, Barcelona, Spain (C. Muñoz-Almagro, C. Esteva, S. Hernandez-Bou, J.J. Garcia-Garcia); Spanish Reference Laboratory for Pneumococci, Madrid, Spain (D. Tarrago); Carlos de Haya Children’s Hospital, Malaga, Spain (D. Moreno-Perez); University of Oxford, Oxford, UK (S.S. Dhillon, A.B. Brueggemann);; GlaxoSmithKline Biologicals, Rixensart, Belgium (W.P. Hausdorff);

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Figure 2

Microbiologic characteristics of pleural fluid (PF) specimens from pediatric parapneumonic empyema (PPE) case-patients. *Streptococcus pyogenes (6), Staphylococcus aureus (3), Mycobacterium tuberculosis (2), Escherichia coli (1), Streptococcus mitis (1), Peptostreptococcus spp. (1). †Pleural fluids analyzed by PCR included 2 samples that were ply negative but wzg positive. ‡18 partially genotyped by multilocus sequence typing (MLST) (>3 alleles), as DNA concentration was too low for reliable PCR amplification and sequencing.

Figure 2. Microbiologic characteristics of pleural fluid (PF) specimens from pediatric parapneumonic empyema (PPE) case-patients. *Streptococcus pyogenes (6), Staphylococcus aureus (3), Mycobacterium tuberculosis (2), Escherichia coli (1), Streptococcus mitis (1), Peptostreptococcus spp. (1). †Pleural fluids analyzed by PCR included 2 samples that were ply negative but wzg positive. ‡18 partially genotyped by multilocus sequence typing (MLST) (>3 alleles), as DNA concentration was too low for reliable PCR amplification and sequencing.

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