Volume 14, Number 9—September 2008
Research
Excretion of Transmissible Spongiform Encephalopathy Infectivity in Urine
Luisa Gregori, Gabor G. Kovacs, Irina Alexeeva, Herbert Budka, and Robert G. Rohwer
Table 1
Titer of urine from scrapie-infected hamsters
| Hamster | Volume assayed, mL | Fold dilution | Volume inoculated, mL | Total no. hamsters | No. infected hamsters | Titer, ID/mL* | SD† |
|---|---|---|---|---|---|---|---|
| Infected | 4.87 | 3 | 14.6 | 292 | 18 | 3.8 | 0.9 |
| Noninoculated | 2 | None | 2 | 40 | 1 | – | – |
*ID, infectious dose. Titer = –ln(P(0)) × (1/v), where P(0) = (noninfected animals)/(total animals inoculated) and v = inoculation volume, 0.05 mL.
†SD = square root (titer/V), where V = 4.87 mL, the total volume of the undiluted urine inoculated (1).
