Emerging Infectious Disease ISSN: 1080-6059

Bluetongue Virus Serotype 8 Reemergence in Germany, 2007 and 2008

Bernd Hoffmann, Michael Saßerath, Sabine Thalheim, Claudia Bunzenthal, Günter Strebelow, and Martin Beer

Author affiliations: Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (B. Hoffmann, G. Strebelow, M. Beer); State Veterinary Institute Krefeld, Krefeld, Germany (M. Saßerath, C. Bunzenthal); Landeslabor Brandenburg, Frankfurt (Oder), Germany (S. Thalheim);

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Figure

A) Bluetongue virus (BTV) serotype 8 genome in a field sample collected May 3, 2007 (BH95/07), detected by using real-time reverse transcription–PCR with a 6-carboxy fluorescein-labeled probe (FAM). The magnitudes of the fluorescence signals per PCR cycle are shown for the sample (Unknown), the no-template control (NTC), and the positive control (PC). Ct, cycle threshold. B) Confirmation of serotype 8 for the isolated virus by serotype-specific PCR and agarose gel analysis. Additionally tested serotypes (BTV 1, 2, 4, 9, 16) were negative. A DNA ladder was used as marker (M).

Figure. A) Bluetongue virus (BTV) serotype 8 genome in a field sample collected May 3, 2007 (BH95/07), detected by using real-time reverse transcription–PCR with a 6-carboxy fluorescein-labeled probe (FAM). The magnitudes of the fluorescence signals per PCR cycle are shown for the sample (Unknown), the no-template control (NTC), and the positive control (PC). Ct, cycle threshold. B) Confirmation of serotype 8 for the isolated virus by serotype-specific PCR and agarose gel analysis. Additionally tested serotypes (BTV 1, 2, 4, 9, 16) were negative. A DNA ladder was used as marker (M).

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