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Volume 14, Number 9—September 2008

Dispatch

Bluetongue Virus Serotype 8 Reemergence in Germany, 2007 and 2008

Bernd Hoffmann, Michael Saßerath, Sabine Thalheim, Claudia Bunzenthal, Günter Strebelow, and Martin BeerComments to Author 
Author affiliations: Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (B. Hoffmann, G. Strebelow, M. Beer); State Veterinary Institute Krefeld, Krefeld, Germany (M. Saßerath, C. Bunzenthal); Landeslabor Brandenburg, Frankfurt (Oder), Germany (S. Thalheim);

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Table

Results of rRT–PCR and bluetongue virus competition ELISA of samples from experimentally inoculated animals*

Day postinoculation Animal no., dose
415, 40 mL IV and 10 mL SC
418, 40 mL IV and 10 mL SC
419, 20 μL SC
rRT-PCR, Ct ELISA rRT-PCR, Ct ELISA rRT-PCR, Ct ELISA
2 >42.0 >42.0 >42.0
4 37.7 37.2 >42.0
6 32.1 28.7 >42.0
9 30.9 26.6 >42.0
13 24.3 22.9 ? >42.0
16 24.3 22.8 + >42.0
21 26.7 24.5 + >42.0
30 27.0 + 27.2 + >42.0

*rRT-PCR, real-time reverse–transcription PCR; IV, intravenous; SC, subcutaneous; Ct, cycle threshold (Ct values >42 were defined as negative); –, negative; +, positive; ?, in doubt.

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