Volume 14, Number 9—September 2008
Dispatch
Bluetongue Virus Serotype 8 Reemergence in Germany, 2007 and 2008
Bernd Hoffmann, Michael Saßerath, Sabine Thalheim, Claudia Bunzenthal, Günter Strebelow, and Martin Beer
Table
Results of rRT–PCR and bluetongue virus competition ELISA of samples from experimentally inoculated animals*
| Day postinoculation | Animal no., dose |
|||||||
|---|---|---|---|---|---|---|---|---|
| 415, 40 mL IV and 10 mL SC |
418, 40 mL IV and 10 mL SC |
419, 20 μL SC |
||||||
| rRT-PCR, Ct | ELISA | rRT-PCR, Ct | ELISA | rRT-PCR, Ct | ELISA | |||
| 2 | >42.0 | – | >42.0 | – | >42.0 | – | ||
| 4 | 37.7 | – | 37.2 | – | >42.0 | – | ||
| 6 | 32.1 | – | 28.7 | – | >42.0 | – | ||
| 9 | 30.9 | – | 26.6 | – | >42.0 | – | ||
| 13 | 24.3 | – | 22.9 | ? | >42.0 | – | ||
| 16 | 24.3 | – | 22.8 | + | >42.0 | – | ||
| 21 | 26.7 | – | 24.5 | + | >42.0 | – | ||
| 30 | 27.0 | + | 27.2 | + | >42.0 | – | ||
*rRT-PCR, real-time reverse–transcription PCR; IV, intravenous; SC, subcutaneous; Ct, cycle threshold (Ct values >42 were defined as negative); –, negative; +, positive; ?, in doubt.
