Figure 2. Phylogenetic relationships in the P1 and viral protein 1 (VP1) genes. Analysis was done by using a neighbor-joining method with pairwise deletion for gaps, and 1,000 bootstrap reiterations for confidence testing. Bootstrap confidence values are depicted next to root points. Branch lengths are proportional to the evolutionary distances used to infer the phylogenetic tree. Phylogenetic analyses were conducted by using MEGA version 4 (24). For cardiovirus isolates from GenBank, accession number is shown after isolate identification number. For economic reasons, only for VP1 is the whole cardiovirus genus depicted. New strains from this study are shown in boldface. TMEV, Theiler murine encephalomyelitis virus; EMCV, murine encephalomyocarditis virus. Scale bars indicate number of substitutions per site.