Volume 15, Number 1—January 2009
Dispatch
Novel Human Rotavirus Genotype G5P[7] from Child with Diarrhea, Cameroon
Mathew D. Esona1
, Annelise Geyer1, Krisztian Banyai, Nicola Page1, Maryam Aminu1, George E. Armah1, Jennifer Hull, Duncan A. Steele1, Roger I. Glass, and Jon R. Gentsch
, Annelise Geyer1, Krisztian Banyai, Nicola Page1, Maryam Aminu1, George E. Armah1, Jennifer Hull, Duncan A. Steele1, Roger I. Glass, and Jon R. GentschTable 1
Primers used for amplification and sequencing of rotavirus genes
| Primer* | Sequence (5′ → 3′)† | Gene‡ | Nucleotide position, strand | Amplicon size, bp | Strain | Reference |
|---|---|---|---|---|---|---|
| MDEVP1F | AAT CAC AAT CTG CAG TTC AAA | VP1 | 68–89, + | 337 | Ku | This article |
| MDEVP1R | AAT GAA TCA GTG TAT TCT TCG | VP1 | 405–384, – | Ku | This article | |
| MDEVP2F | CTG ACA AAG TGC TAT CAC A | VP2 | 156–175, + | 300 | Ku | This article |
| MDEVP2R | AGG TAA TTG TCT TGG TTC | VP2 | 456–438, – | Ku | This article | |
| MDEVP3F | TTG CTA GAT TGT CAA ATC GTG | VP3 | 597–618, + | 327 | Ku | This article |
| MDEVP3R | AAT AAG ATG GAG CTG AAC C | VP3 | 924–905, – | Ku | This article | |
| MDENSP1F | GAG ACC RTC AAC TCC TAC YAA | NSP1 | 120–141, + | 344 | Wa | This article |
| MDENSP1R | ATT GTA AYG TTA TTG GCA T | NSP1 | 464–445, – | Wa | This article | |
| MDENSP2F | GCT TGC TTT TGT TAT CCT | NSP2 | 58–76, + | 327 | Ku | This article |
| MDENSP2R | ATT TTC CAA ATG TCT AAC AG | NSP2 | 385–365, – | Ku | This article | |
| MDENSP3F | GCC ACT TCA ACA TTA GAA | NSP3 | 101–119, + | 303 | Ku | This article |
| MDENSP3R | TAC ACT AAA ACA AGC ATT AAG | NSP3 | 404–383, – | Ku | This article | |
| MDENSP5F | AGC GCT ACA GTG ATG TCT CT | NSP5 | 10–29, + | 337 | Ku | This article |
| MDENSP5R | CCA TTT GAT CGC ACC CA | NSP5 | 347–330, – | Ku | This article | |
| JRG30 | GGC TTT TAA AAG TTC TGT T | NSP4 | 1–19, + | 737 | Wa | This article |
| JRG31 | ACC ATT CCT TCC ATT AAC | NSP4 | 738–721, – | Wa | This article | |
| Con3 | TGG CTT CGC TCA TTT ATA GAC A | VP4 | 11–32, + | 876 | Ku | (5) |
| Con2 | ATT TCG GAC CAT TTA TAA CC | VP4 | 887–868, – | Ku | (5) | |
| 9con1-L | TA GCT CCT TTT AAT GTA TGG TAT | VP7 | 37–59, + | 896 | Wa | Modified from (6) |
| VP7-Rdeg | GAC GGV GCR ACT ACA TGG T | VP7 | 933–914, – | Wa | Modified from (7) | |
| VP6-F | GAC GGV GCR ACT ACA TGG T | VP6 | 747–766, + | 379 | (8) | |
| VP6-R | GTC CAA TTC ATN CCT GGT G | VP6 | 1126–1106, – | (8) |
*F, forward; R, reverse.
†R, A or G; Y, C or T; V, A, C, or G; N, A, C, G, or T.
‡VP, structural protein; NSP, nonstructural protein.
