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Volume 15, Number 10—October 2009

Research

Discriminatory Ability of Hypervariable Variable Number Tandem Repeat Loci in Population-based Analysis of Mycobacterium tuberculosis Strains, London, UK

Preya Velji1, Vladyslav Nikolayevskyy1, Timothy Brown, and Francis DrobniewskiComments to Author 
Author affiliations: Barts and The London School of Medicine, Queen Mary, University of London, London, UK (P. Velji, V. Nikolayevskyy); Health Protection Agency National Mycobacterium Reference Laboratory, London (T. Brown, F. Drobniewski); 1These authors contributed equally to this article.

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Table 2

Number of DNA extracts (from n = 16) for which peaks were detected by different conditions for capillary electrophoresis of Mycobaterium tuberculosis after amplifying the loci with different polymerases, London, UK, 2005–2006*

Locus Method† Bioline polymerases‡
QIAGEN polymerases‡
BIOTAQ Diamond HotStartTaq HotStartTaq Plus
MIRU 26 1 16 16 16 (1) 16 (1)
2 15 16 16 (1) 16 (1)

3
16
16

16
16
ETR-B 1 16 16 16 (1) 16 (1)
2 15 16 16 (1) 16 (1)

3
16
16

16 (2)
16 (2)
VNTR 1982 1 8 13 14 14
2 9 13 12 14

3
6
11

12
14
VNTR 3232 1 11 15 13 14
2 10 15 14 14
3 11 (3) 15 (7) 13 (6) 13 (4)

*MIRU, mycobacterial interspersed repetitive unit; ETR, exact tandem repeat; VNTR, variable number tandem repeats.
†Refer to Table 1.
‡Numbers in parentheses represent number of extracts whose calculated number of repeats were higher and lower than the expected value on the basis of that produced by the standard procedure (method 1).

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