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Volume 15, Number 2—February 2009

Research

Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Bart J. CurrieComments to Author , Asha Haslem, Talima Pearson, Heidie Hornstra, Benjamin Leadem, Mark Mayo, Daniel Gal, Linda Ward, Daniel Godoy, Brian G. Spratt, and Paul Keim
Author affiliations: Menzies School of Health Research, Darwin, Northern Territory, Australia (B.J. Currie, A. Haslem, M. Mayo, D. Gal, L. Ward); Northern Territory Clinical School, Darwin (B.J. Currie, L. Ward); Northern Arizona University, Flagstaff, Arizona, USA (T. Pearson, H. Hornstra, B. Leadem, P. Keim); Imperial College, London, UK (D. Godoy, B.G. Spratt)

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Table 2

Fragment size and repeat copy number (MLVA-4 code) for 6 Burkholderia pseudomallei strains used as MLVA-4 standards*

Strain VNTR loci
2341
 389
 1788
 933
Size, bp† Repeat copy no. Size, bp† Repeat copy no. Size, bp† Repeat copy no. Size, bp† Repeat copy no.
MSHR978 189.85 11 236.19 8 265.63 6 254.8 10
MSHR1822 190.25 11 245.02 9 282.56 7 290.55 13
MSHR114 145.49 6 252.32 10 298.4 8 242.57 9
MSHR1641 154.5 7 236.22 8 315.39 9 230.81 8
MSHR1153 127.93 4 236.24 8 298.51 8 194.96 5
MSHR1123 172.34 9 260.28 11 331.48 10 218.91 7

*MLVA-4, 4-locus multilocus variable number tandem repeat analysis.
†Error range in fragment sizing is ± 3 bp.

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