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Volume 15, Number 2—February 2009

Research

Characteristics of 263K Scrapie Agent in Multiple Hamster Species

Kimberly D. Meade-WhiteComments to Author , Kent D. Barbian, Brent Race, Cynthia Favara, Don Gardner, Lara Taubner, Stephen Porcella, and Richard Race
Author affiliations: National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA

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Figure 3

A) Proteinase K–resistant prion protein (PrPres) glycoform profiles for 6 hamster species for each of 3 successive passages: 1, initial cross-species passage; 2, second intraspecies passage; 3, third intraspecies passage. Each passage represents 6 different animals, each quantified 6–8 times. Each lane had 0.5 mg tissue equivalents per lane. ●, percentage of unglycosylated band; ■, partially glycosylated band; ▲, fully glycoslyated band. Western blot representation of glycoform for each species

Figure 3. A) Proteinase K–resistant prion protein (PrPres) glycoform profiles for 6 hamster species for each of 3 successive passages: 1, initial cross-species passage; 2, second intraspecies passage; 3, third intraspecies passage. Each passage represents 6 different animals, each quantified 6–8 times. Each lane had 0.5 mg tissue equivalents per lane. ●, percentage of unglycosylated band; ■, partially glycosylated band; ▲, fully glycoslyated band. Western blot representation of glycoform for each species visualized using R30 and enhanced chemifluorescence. Error bars indicate SEM. B) Serially passaged 263K scrapie from 5 hamster species passaged back to Syrian hamster. Western blot analysis of clinically ill Syrian hamster infected with Syrian 263K or 263K passaged 3 times through the new hamster host. Syrian hamster inoculated with brain homogenate from the following hamsters: lane 1, Syrian 263K; 2, Turkish; 3, Chinese; 4, Armenian; 5, Djungarian; 6, Siberian. Tissue equivalents: lane 1, 0.5 mg; lanes 2, 5, and 6, 0.4 mg; lane 3, 0.7 mg; and lane 4, 0.9 mg.

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