Figure 3. A) Proteinase K–resistant prion protein (PrPres) glycoform profiles for 6 hamster species for each of 3 successive passages: 1, initial cross-species passage; 2, second intraspecies passage; 3, third intraspecies passage. Each passage represents 6 different animals, each quantified 6–8 times. Each lane had 0.5 mg tissue equivalents per lane. ●, percentage of unglycosylated band; ■, partially glycosylated band; ▲, fully glycoslyated band. Western blot representation of glycoform for each species visualized using R30 and enhanced chemifluorescence. Error bars indicate SEM. B) Serially passaged 263K scrapie from 5 hamster species passaged back to Syrian hamster. Western blot analysis of clinically ill Syrian hamster infected with Syrian 263K or 263K passaged 3 times through the new hamster host. Syrian hamster inoculated with brain homogenate from the following hamsters: lane 1, Syrian 263K; 2, Turkish; 3, Chinese; 4, Armenian; 5, Djungarian; 6, Siberian. Tissue equivalents: lane 1, 0.5 mg; lanes 2, 5, and 6, 0.4 mg; lane 3, 0.7 mg; and lane 4, 0.9 mg.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.