Volume 15, Number 3—March 2009
Research
Shiga Toxin–producing Escherichia coli Strains Negative for Locus of Enterocyte Effacement
Hayley J. Newton1, Joan Sloan1, Dieter M. Bulach, Torsten Seemann, Cody C. Allison, Marija Tauschek, Roy M. Robins-Browne, James C. Paton, Thomas S. Whittam, Adrienne W. Paton, and Elizabeth L. Hartland
Table 2
Gene names, plasmid location, and oligonucleotide sequences used to examine the prevalence of pO113-encoded genes among STEC strains*
| Gene | Plasmid location, bp | Oligonucleotide sequences | Amplicon size, bp | PCR annealing temperature, °C |
|---|---|---|---|---|
| pilQ | 9593–12105 | F: 5’-TTGCAGACCCGCAGTTG-3’ R: 3’-CAGGGCTTCGGCGATGT-5’ | 870 | 52 |
| epeA | 48716–52795 | F:5’-CAGGTGGTACTGTCGGC-3’ R: 3’-GCCCATGCCGCTCTGAA-5’ | 667 | 46 |
| trbC | 57357–59660 | F: 5’-GCCACCACCGGTGGCGG-3’ R: 3’-CAATCAGAATGCGGTCG-5’ | 230 | 50 |
| repA | 106112–107089 | F: 5’-AAAGTCTTGTATAGCTC-3’ R: 3’-GTTATCCATATCCAGGC-5’ | 871 | 44 |
| ehxA | 114136–117132 | F: 5’-CCCAGGAGAAGAAGTCA-3’ R: 3’-CTTCACCTGAGGCATCTT-5’ | 1,108 | 48 |
| espP | 134205–140707 | F: 5’-AAACAGCAGGCACTTGAACG-3’ R: 3’-GGAGTCGTCAGTCAGTAGAT-5’ | 2,000 | 52 |
| iha | 146066–149549 | F: 5’-TCCAGTCAGTACCACGA-3’ R: 3’-CTGTCGGAAAGTTTCAC-5’ | 981 | 48 |
| subAB | 150678–152419 | F: 5’-GTGTACAGGACTCATGG-3’ R: 3’-ATCACCAGTCCACTCAG-5’ | 783 | 48 |
| repZ | 163918–164949 | F: 5’-ATACAGGAGTAAAACCG-3’ R: 3’-CATATAACGCAGTACAC-5’ | 1,792 | 46 |
*STEC, Shiga toxin–producing Escherichia coli; F, forward primer; R, reverse primer.
