Fabrizio Carletti, Licia Bordi, Concetta Castilletti, Antonino Di Caro, Laura Falasca, Cristiana Gioia, Giuseppe Ippolito, Stefania Zaniratti, Anna Beltrame, Pierluigi Viale, and Maria Rosaria Capobianchi
Author affiliations: National Institute for Infectious Diseases “L. Spallanzani,” Rome, Italy (F. Carletti, L. Bordi, C. Castilletti, A. Di Caro, L. Falasca, C. Gioia, G. Ippolito, S. Zaniratti, M.R. Capobianchi); University of Udine, Udine, Italy (A. Beltrame, P. Viale)
Figure. Phylogenetic tree of nucleotide sequences of the complete hemagglutinin open reading frame (930 bp) of orthopoxviruses (OPVs) isolated from the 2 patients described in this report and additional poxviruses available in GenBank (cowpox: AY902279, AY902269, AF375085, AF377883, AF377886, AY902262, AY944028, Z99047, AY366477, AF377877, AF375087; taterapox: AF375093; camelpox: AY902250, DQ853384; horsepox: DQ792504; elephantpox: AF375090; vaccinia: AY902305, X91135, AF375078, AF375077; rabbitpox: AF375119, AF375118, Z99049; variola: AF375129, AF375130, AF375138, AF375142; ectromelia: AY902302, AY902303, AY902306; monkeypox: DQ443504, AF375105, AF375112). Multiple alignment was generated with ClustalW 1.7 software in BioEdit (www.mbio.ncsu.edu/BioEdit/BioEdit.html), and the phylogenetic tree was constructed by using maximum-likelihood and neighbor-joining algorithms implemented in Mega 4.0 software (www.megasoftware.net). Bootstrap values >75 are shown at nodes. The sequences from the patients described in this report form a distinct cluster separate from other OPV sequences. Scale bar indicates genetic distance.
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