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Volume 15, Number 3—March 2009

Dispatch

Coordinated Implementation of Chikungunya Virus Reverse Transcription–PCR

Marcus Panning, Remi N. Charrel, Oliver D. Mantke, Olfert Landt, Matthias Niedrig, and Christian DrostenComments to Author 
Author affiliations: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (M. Panning); Université de la Méditerranée, Marseille, France (R.N. Charrel); Robert Koch Institute, Berlin, Germany (O.D. Mantke, M. Niedrig); TIB MOLBIOL, Berlin (O. Landt); University of Bonn Medical Centre, Bonn, Germany (C. Drosten)

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Table 2

Possible technical factors influencing performance of laboratories in detection of CHIKV*

Factor No. laboratories p value for positive influence on sensitivity
QIAGEN† viral RNA extraction kit 23 0.2
Any automated RNA extraction procedure 8 0.08
Preformulated CHIKV real-time RT-PCR protocol 13 0.03
Any real-time CHIKV RT-PCR 27 0.3
Any nested CHIKV RT-PCR 6 0.37

*CHIKV, chikungunya virus; RT-PCR, reverse transcription–PCR.
†Hilden, Germany.

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