Volume 15, Number 3—March 2009
Research
Integron-mediated Multidrug Resistance in a Global Collection of Nontyphoidal Salmonella enterica Isolates
Mary G. Krauland, Jane W. Marsh, David L. Paterson, and Lee H. Harrison
Table 2
Primers used for PCR amplification of Salmonella enterica integrons
| Primer | Sequence (5′ → 3′) | Target | Reference |
|---|---|---|---|
| 5’CS | GGCATCCAAGCAGCAAGC | 5′ conserved segment | (17) |
| 3’CS | AAGCAGACTTGACCTGAT | 3′ conserved segment | (17) |
| int_F | CGATGCGTGGAGACCGAAACCTT | intI1 | (18) |
| int_R | GTAACGCGCTTGCTGCTTGGATGC | intI1 | (18) |
| invA_F | ACACAGCTCGTTTACGACCTGAAT | invA | (16) |
| invA_R | AGACGACTGGTACTGATCGATATT | invA | (16) |
| sul1_F | GCGCGGCGTGGGCTACC | sul1 | This study |
| sul1_R | CCGCAAGGCTCGCTGGAC | sul1 | This study |
| aadA1_R | CGATGACGCCAACTACCTCTGATA | aadA1internal primer | This study |
| arr2_F | ATTGTTGGCGTTGTTGAAGACTGG | arr2 internal primer | This study |
| cmlA5_F | GAATGGGAATGGGATGCCTGATAG | cmlA5 internal primer | This study |
| oxa10_R | TTTACAAAGCACGAAGACACCATT | blaOXA10 internal primer | This study |
| cmlA_F | GCAGGTCGCGAGGAAAGTAATG | cmlA 5′ forward primer | This study |
| cmlA_R | ACACCGCCCAAGCAGAAGTAGA | cmlA 3′ reverse primer | This study |
| blaOXA30_F | TCGCAAGAAATAACCCAAAAA | blaOXA30 internal primer | This study |
| aacA4_F | AAGCGGGGTTTGAGAGG | aacA4 forward primer | This study |
| aacA4_R | CGCGTACTCCTGGATCGGTTTCTT | aacA4 reverse primer | This study |
| dfr_1_F | TTTAGGCCAGTTTTTACCCAAGAC | dfrA1 internal primer | This study |
| ere_est_R | GCGCCAGCAGAATTATCCTTACAT | ereA2 internal primer | This study |
| aac(6’)IIC_F | CCGCGGGATTGACCAGT | aac(6′)IIC internal primer | This study |
| dfrA12_F | GCTGCGCATTTTGGTTCC | dfrA12 internal primer | This study |
| aadA2_R | TGTCATTGCGCTGCCATTCTCC | aadA2 internal primer | This study |
| qacH_F | GCGTCGCCGTTCTAAATCTGCTAT | qacH internal primer | This study |
| aac_R | GGGCGCCGGGTGTCTGGAG | aacA4 internal primer | This study |
| IS_F | GTCACGCCCCGACCATCACCTTCC | IS1247 internal primer | This study |
| TNP_F | CCGCGCTGGCCGACCTGAAC | Transposase A internal primer | This study |
| ere_F | CCTAACCGGGCGATTCAA | Erythromycin esterase internal primer | This study |
| cmlA_R_internal | ATCACACGCCCCATAAAACGAG | cmlA internal primer | This study |
| arr_R2 | GCGGGATCCAGAACCAGGCGACAT | arr-2 internal primer | This study |
| arr_accA_R | AGAGCGGCTTTGGTTCC | Internal primer arr-2–accA junction | This study |
| ere_F2 | CGCTGATTTCGCTGTCCTGA | ereA internal primer | This study |
| dfrA17_F | AAAAAGGCTAACAAGTCGT | dfrA17 internal primer | This study |
| cml_R2 | GCTGAATTGTGCTCGCTGTCGTA | cml internal primer | This study |
| aadA_con_F | CGACATCATYCCGTGGCGTTAT | aadA forward consensus primer | This study |
| aadA_con_R | CGGCAGCCACATCCTTC | aadA reverse consensus primer | This study |
| aacA4_F | ATGACCTTGCGATGCTCT | aacA4 internal primer | This study |
| aacA4_R | CTCGATGGAAGGGTTAGG | aacA4 internal primer | This study |
| blaOXA30_F | ACACAATACATATCAACTTCGC | blaOXA30-aadA internal primer | This study |
| aadA1_R_S | GGATAACGCCACGGAATGATGTC | aadA1 internal primer | This study |
| albany_PSE1a_F | CCTTTGGGGCCACCTACAG | blaPSE1 primer | This study |
| albany_PSE1b_F | ATCAAAATTATGGGGTTACTTACA | blaPSE1 primer | This study |
| albany_dfr1_F | ATGGTAGCTATATCGAAGAATGGA | dfr primer | This study |
| albany_dfr2_F | AAGTACTGGCTATTGCCTTAGGAG | dfr primer | This study |
| U7-L12 | ACACCTTGAGCAGGGCAAAG | SGI1 left junction | (15) |
| LJ-R1 | AGTTCTAAAGGTTCGTAGTCG | SGI1 left junction | (15) |
| 104-RJ | TGACGAGCTGAAGCGAATTG | SGI1 right junction | (15) |
| 104-D | ACCAGGGCAAAACTACACAG | SGI1 right junction | (15) |
