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Volume 15, Number 4—April 2009

Letter

Mycobacterium avium subsp. hominissuis Infection in a Pet Parrot

Edmealem Jembere Shitaye, Veronika Grymova, Martin Grym, Roman Halouzka, Alica Horvathova, Monika Moravkova, Vladimir Beran, Jana Svobodova, Lenka Dvorska-Bartosova, and Ivo PavlikComments to Author 
Author affiliations: Veterinary Research Institute, Brno, Czech Republic (E.J. Shitaye, A. Horvathova, M. Moravkova, V. Beran, L. Dvorska-Bartosova, I. Pavlik); University of Veterinary and Pharmaceutical Sciences, Brno (E.J. Shitaye, R. Halouzka); Veterinary Clinic AvetuM, Brno (V. Grymova, M. Grym); Regional Institute of Public Health, Brno (J. Svobodova)

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Table

Detection of Mycobacterium avium subsp. hominissuis in a female blue-fronted Amazon parrot (Amazona aestiva)*

Origin of examined
tissue samples Mycobacteria detection
PCR†
IS1245
RFLP§
ZN Culture‡ IS1245 IS901
Lung + (3) + a
Kidney
Heart
Liver + + (15) + a
Intestine + + (2) + b
Stomach + (8) + a
Bone marrow + (1) + a
Brain
Musculus pectoralis + (34) + a

*ZN, Ziehl-Neelsen microscopy of homogenate for acid-fast rods; RFLP, restriction fragment length polymorphism; –, not detected/negative; +, detected/positive.
†PCR for IS1245 and IS901 was carried out according to the method described in (7).
‡Culture examination performed as described by Matlova et al. (6); colony-forming units per isolation are shown in parentheses.
§Standardized IS1245 RFLP method was performed according to Van Soolingen et al. (8).

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