Volume 15, Number 4—April 2009
Letter
Mycobacterium avium subsp. hominissuis Infection in a Pet Parrot
Edmealem Jembere Shitaye, Veronika Grymova, Martin Grym, Roman Halouzka, Alica Horvathova, Monika Moravkova, Vladimir Beran, Jana Svobodova, Lenka Dvorska-Bartosova, and Ivo Pavlik
Table
Detection of Mycobacterium avium subsp. hominissuis in a female blue-fronted Amazon parrot (Amazona aestiva)*
| Origin of examined tissue samples | Mycobacteria detection |
PCR† |
IS1245 RFLP§ | |||
|---|---|---|---|---|---|---|
| ZN | Culture‡ | IS1245 | IS901 | |||
| Lung | – | + (3) | + | – | a | |
| Kidney | – | – | ||||
| Heart | – | – | ||||
| Liver | + | + (15) | + | – | a | |
| Intestine | + | + (2) | + | – | b | |
| Stomach | – | + (8) | + | – | a | |
| Bone marrow | – | + (1) | + | – | a | |
| Brain | – | – | ||||
| Musculus pectoralis | – | + (34) | + | – | a | |
*ZN, Ziehl-Neelsen microscopy of homogenate for acid-fast rods; RFLP, restriction fragment length polymorphism; –, not detected/negative; +, detected/positive.
†PCR for IS1245 and IS901 was carried out according to the method described in (7).
‡Culture examination performed as described by Matlova et al. (6); colony-forming units per isolation are shown in parentheses.
§Standardized IS1245 RFLP method was performed according to Van Soolingen et al. (8).
