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Volume 15, Number 4—April 2009

Dispatch

High Prevalence of Spirochetosis in Cholera Patients, Bangladesh

Eric J. Nelson, Angela Tanudra, Ashrafuzzaman Chowdhury, Anne V. Kane, Firdausi Qadri, Stephen B. Calderwood, Jenifer Coburn, and Andrew CamilliComments to Author 
Author affiliations: Howard Hughes Medical Institute–Tufts University School of Medicine, Boston, Massachusetts, USA (E.J. Nelson, A. Camilli); Medical College of Wisconsin, Milwaukee, Wisconsin, USA (A. Tanudra, J. Coburn); Tufts Medical Center, Boston (A. Tanudra, A.V. Kane, J. Coburn); Jahangirnagar University, Dhaka, Bangladesh (A. Chowdhury); International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka (F. Qadri); Massachusetts General Hospital, Boston (S.B. Calderwood); Harvard Medical School, Boston (S.B. Calderwood)

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Figure

Neighbor-joining (NJ) phylogeny of NADH oxidase (nox) sequences of Brachyspira pilosicoli from 5 cholera patients (A–E). The nox sequences were PCR amplified, cloned, and sequenced from each patient (individual clones are appended _SX). Published sequences from known species are included for reference. NJ analysis was performed by using an NJ model and 1,000 bootstraps. Bootstrap values >800 are presented next to nodes. The scale bar indicates a 2% bp change (contiguous sequence ≈990 bp).

Figure. Neighbor-joining (NJ) phylogeny of NADH oxidase (nox) sequences of Brachyspira pilosicoli from 5 cholera patients (A–E). The nox sequences were PCR amplified, cloned, and sequenced from each patient (individual clones are appended _SX). Published sequences from known species are included for reference. NJ analysis was performed by using an NJ model and 1,000 bootstraps. Bootstrap values >800 are presented next to nodes. The scale bar indicates a 2% bp change (contiguous sequence ≈990 bp).

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