Volume 15, Number 5—May 2009
Research
Virulent Strain of Hepatitis E Virus Genotype 3, Japan
Kazuaki Takahashi
, Hiroaki Okamoto, Natsumi Abe, Manri Kawakami, Hiroyuki Matsuda, Satoshi Mochida, Hiroshi Sakugawa, Yoshiki Suginoshita, Seishiro Watanabe, Kazuhide Yamamoto, Yuzo Miyakawa, and Shunji Mishiro
, Hiroaki Okamoto, Natsumi Abe, Manri Kawakami, Hiroyuki Matsuda, Satoshi Mochida, Hiroshi Sakugawa, Yoshiki Suginoshita, Seishiro Watanabe, Kazuhide Yamamoto, Yuzo Miyakawa, and Shunji MishiroTable 2
Amino acid residues in 8 human and 5 swine hepatitis E virus isolates compared with those in the other genotype 3 isolates*
| Amino acid position† | Conserved in genotype 3 | Human no. |
Swine no. |
Conserved in genotype 4 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 1 | 2 | 3 | 4 | 5 | ||||
| ORF1 | ||||||||||||||||
| 154 | A | A | T | A | A | T | T | A | T | A | A | T | A | T | T | |
| 547 | R | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | R | |
| 598 | R | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | K | |
| 605 | S | P | P | P | P | P | P | P | P | P | P | P | P | P | P | |
| 721 | A | T | T | T | T | T | T | T | T | T | T | T | T | T | A | |
| 807 | A | S | S | S | S | S | S | S | S | S | S | S | S | S | A | |
| 978 | I | V | V | V | V | V | V | V | V | V | V | V | V | V | V | |
| 979 | S | K | K | K | K | K | K | K | K | K | K | K | K | K | E | |
| 1135 | I | T | T | T | T | T | T | T | T | T | T | T | T | T | V | |
| 1213‡ | V | V | A | A | A | A | A | A | A | A | A | A | A | A | A | |
| 1246 | Q | H | H | H | H | H | H | H | H | H | H | H | H | H | D | |
| 1469 |
C |
S |
S |
S |
S |
S |
S |
S |
S |
S |
S |
S |
S |
S |
C |
|
| ORF2 | ||||||||||||||||
| 98 | P | S | S | P | P | S | S | P | P | P | P | S | P | S | A | |
| 113 | V/I | T | T | T | T | T | T | T | T | T | T | T | T | T | V | |
| 660 |
S |
S |
S |
S |
F |
F |
F |
S |
F |
S |
S |
S |
S |
S |
Y |
|
| ORF3 | ||||||||||||||||
| 91 | S | N | N | N | N | N | N | N | N | N | N | N | N | N | S | |
| 97 | A | A | V | V | V | V | V | V | V | V | V | V | V | V | A | |
| 98 | P | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | P | |
*Eighteen amino acids of 8 human isolates (JIO strain) and 5 swine isolates (swJ19 strain) not shared by other genotype 3 isolates. The 3 at positions 605, 978, and 1213 (boldface) were the same as the corresponding residues in genotype 4 isolates.
†Corresponds to the position in hepatitis E virus (HEV)-US2 (GenBank/EMBL/DDBJ accession no, AF060669) (12).
‡V1213A in the open reading frame (ORF) 1 polyprotein corresponds to V239A in the HEV-US2 genotype 3 isolate helicase domain within ORF1 (online Appendix Figure.
