Volume 15, Number 5—May 2009
Letter
Klebsiella pneumoniae Carbapenemase, Canada
, Roberto Melano, Prasad Rawte, Stephen Lo, Nathalie Tijet, Milan Fuksa, Nancy Roda, David J. Farrell, and Sigmund KrajdenTable
Results of initial susceptibility and supplementary testing for Klebsiella pneumoniae carbapenemase in urine and sputum samples from 73-year-old man, Canada*
| Isolate | MIC, µg/mL† |
Disk diffusion results, mm |
Initial report‡ | Final report§ | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMP | FOX | CIP | GEN | CTRX | MEM | FOX | CAZ | CAC | CTX | CTC | ||||
| 7184 | >16 | >16 | >2 | 8 | >32 | 8 | 16 | 0 | 14 | 13 | 15 | AmpC/ESBL | KPC | |
| 7315 | >16 | >16 | >2 | 8 | >32 | 4 | 0 | 0 | 8 | 13 | 15 | AmpC/ESBL | KPC | |
*AMP, ampicillin; FOX, cefoxitin; CIP, ciprofloxacin; GEN, gentamicin; CTRX, ceftriaxone; MEM, meropenem; CAZ, ceftazidime; CAC, ceftazidime-clavulanic acid; CTX, cefotaxime; CTC, cefotaxime-clavulanic acid; ESBL, extended-spectrum β-lactamase; KPC, Klebsiella pneumoniae carbapenemase.
†MIC values for clinical isolates 7184 (urine) and 7315 (sputum) were obtained by using agar macrodilution.
‡Initial screening for ESBL or AmpC β-lactamase activity, performed by Kirby Bauer disk diffusion according to Clinical Laboratory Standards Institute guidelines (6,7), suggested ESBL or AmpC β-lactamase activity.
§Supplementary modified Hodge test; PCR (specific for blaKPC family), and DNA sequencing confirmed the presence of KPC activity due to blaKPC-2.
