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Volume 15, Number 7—July 2009

Letter

Ranavirus Outbreak in North American Bullfrogs (Rana catesbeiana), Japan, 2008

Yumi UneComments to Author , Akiko Sakuma, Hiroki Matsueda, Katsuki Nakai, and Masaru Murakami
Author affiliations: Azabu University, Kanagawa, Japan (Y. Ume, A. Sakuma, H. Matsueda, M. Murakami); Nature Conservation Division of Shiga Prefecture, Shiga, Japan (K. Nakai)

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Appendix Figure

North American bullfrog (Rana catesbeiana) metamorphs infected with ranavirus RCV-JP. A) Necrosis of distal extremities (arrows) and mild abdominal swelling. Scale bar = 1 cm. B) Severe abdominal swelling caused by body cavity effusion. Scale bar = 1 cm. C) Kidney of an infected frog with necrosis of glomeruli and tubular hyaline droplet degeneration; hematoxylin and eosin stain. Scale bar = 100  m. Inset shows ranavirus-like particles; scale bar = 100 nm.

Appendix Figure. North American bullfrog (Rana catesbeiana) metamorphs infected with ranavirus RCV-JP. A) Necrosis of distal extremities (arrows) and mild abdominal swelling. Scale bar = 1 cm. B) Severe abdominal swelling caused by body cavity effusion. Scale bar = 1 cm. C) Kidney of an infected frog with necrosis of glomeruli and tubular hyaline droplet degeneration; hematoxylin and eosin stain. Scale bar = 100 m. Inset shows ranavirus-like particles; scale bar = 100 nm.

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