Figure. Phylogenetic analysis of nucleotide sequences of the virus protein 1 (VP1) gene of Saffold cardiovirus. The tree was constructed by using the Molecular Evolutionary Genetics Analysis (MEGA) software version and the neighbor-joining algorithm with kimura-2 parameters (14). The analysis included human Theiler murine encephalomyelitis virus (TMEV)–like cardiovirus. TMEV-like cardiovirus sequences (GenBank accession no. EU376394) and the previously reported SAFV sequences including the prototype SAFV, U2-U7, Can112051-06, BR/118/2006, D/VI2229/2004, D/VI2223/2004, and D/VI2273/2004 (GenBank accession nos. EF165067, NC009448, EU604745-EU604750, AM922293, and EU681176-EU681179) as references. Because the sequences of SAFV-4 to SAFV-8 are not available, they are not included in the phylogenetic tree. Each strain from this study is indicated by a specific identification code (GL) followed by the patient number (GL311, GL317, GL328, GL341, GL352, GL361, GL362, GL365, GL368, GL371, GL376, and GL377) and its GenBank accession number. Scale bar indicates nucleotide substitutions per site.