Enterotoxigenic Escherichia coli Multilocus Sequence Types in Guatemala and Mexico
Matilda Nicklasson , John Klena, Claudia Rodas, August Louis Bourgeois, Olga Torres, Ann-Mari Svennerholm, and Åsa Sjöling
Author affiliations: University of Gothenburg, Gothenburg, Sweden (M. Nicklasson, C. Rodas, A.-M. Svennerholm, Å. Sjöling); United States Naval Medical Research Unit 3, Cairo, Egypt (J. Klena); Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA (A.L. Bourgeois); Instituto de Nutrición de Centro América y Panamá, Guatemala City, Guatemala (O. Torres)
Figure. Dendrogram of the 24 enterotoxigenic Escherichia coli strains from Guatemala and Mexico included in the study, showing multilocus sequence type. Sequences were assembled with BioEdit and aligned using ClustalX within BioEdit (12). The dendogram represents the relationship of a concatenation of the sequences from each strain and was constructed by using MEGA 3.1 (13). Phylogenetic reconstructions were created by using the neighbor-joining method with the Kimura 2-parameter substitution model, using 1,000 bootstrap replicates. A similar arrangement of the strains was indicated by eBURST version 2 analysis (http://eburst.mlst.net). Scale bar indicates dissimilarity, where 0 is completely identical and 1 is completely dissimilar.
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