Volume 16, Number 2—February 2010
Investigating an Airborne Tularemia Outbreak, Germany
|PCR||Culture||Ag detection (LPS)|
|Water||2||Creek, water hose||Neg||Neg||Neg|
|Swabs||16||Game chamber||Neg||Neg (4/4)||–|
|Hare fur, insects||3||Game chamber||Neg||–||–|
|Liquid samples (flush)||9||Game chamber||Neg||–||-|
|Frozen parts of hares received from 9 different households (muscle, bone marrow, fluids recovered during thawing)||14||12–14 hares (Lepus europaeus), shot 2005 Oct 29||Pos 11*/14||Neg (6/6)||Pos. 6†/14|
|Liver/spleen samples||29||15 hares, 1 nutria (Myocastor coypus), shot 2005 Dec 12||Neg||–||–|
|Organs of hares (liver, spleen, whole blood)||72||24 hares, shot 2006 Jan 7 and Jan 14||Neg‡||–||–|
*For all parts, tissue, bone marrow, and fluid from thawing were tested. Samples were considered positive when >2 materials were repeatedly positive by 2 different PCRs. Ag, antigen; LPS, lipopolysaccharide; Neg, negative; Pos, positive.
†Samples also positive by PCR.
‡PCR inhibition noticed for 19/24 blood samples.