Skip directly to search Skip directly to A to Z list Skip directly to site content
CDC Home

Volume 16, Number 2—February 2010

Research

Tropheryma whipplei in Patients with Pneumonia

Sabri Bousbia, Laurent Papazian, Jean-Pierre Auffray, Florence Fenollar, Claude Martin, Wenjun Li, Laurent Chiche, Bernard La Scola, and Didier RaoultComments to Author 
Author affiliations: CNRS-IRD Faculté de Médecine, Marseille, France (S. Bousbia, L. Papazian, F. Fenollar, W. Li, B. La Scola, D. Raoult); Hôpitaux Sud, Marseille (L. Papazian, J.P. Auffray, L. Chiche); Hôpital Nord, Marseille (C. Martin)

Main Article

Table 1

PCR test results of bronchoalveolar lavage specimens that were positive for Tropheryma whipplei DNA, collected from 6 intensive care unit patients in Marseille, France, February 2007–January 2008*

Specimen no.
16S rDNA
for T. whipplei
qPCR probe

Other bacteria identified (16S rDNA primer set)
Twhi3
Ct
Twhi2
Ct
Load (copy)
Bacterium
Identity, %
5 + + 20 + 21 5.105 99
10 + 30 + 31 4,8.102 Streptococcus pneumoniae 99
Actinobacillus pleuropneumoniae 99








Peptostreptococcus sp.
99
82 + 29 + 28 5,3.103 Streptococcus genomosp. C4 99
Uncultured 99
Streptococcus sp. clone 2.17 89
Streptococcus sanguinis 99
Gemella sanguinis 99
Leptotrichia sp. 95
Haemophilus quentini 99
Uncultured Haemophilus sp. 99
Peptostreptococcus sp. 99
Granulicatella para-adiacens 95
Prevotella sp. 99
Prevotella melaninogenica 99
Cloacibacterium normanense 93
Ralstonia solanacearum 99
Haemophilus haemolyticus 99
Uncultured Arcobacter sp. clone DS126 99
Prevotella salivae 99








Streptococcus parasanguis
99
86 + 31 + 29 7,6.102 Uncultured Porphyromonas sp. clone 302E06 99
Uncultured Capnocytophaga sp. 95
Gemella sanguinis 99
Streptococcus constellatus 99
Prevotella melaninogenica 91
Uncultured Porphyromonas sp. 93
Haemophilus parainfluenzae 99
Uncultured Tannerella sp. 98
Prevotella melaninogenica 99








Granulicatella para-adiacens
99
183 + 36 + 37 50 Staphylococcus epidermidis 99
Porphyromonas gingivalis 99
Pseudomonas sp. 92
Eubacterium sp. 94
Streptococcus anginosus 99
Uncultured Neisseria sp. clone 502G08 99
Treponema sp. 97
Clostridium sp. 97
Acidovorax sp./Diaphorobacter sp. 99
Eubacterium brachy 99
Gemella haemolysans 99
Mycoplasma orale 95
Comamonas denitrificans 95
Prevotella tannerae 98








Peptostreptococcus micros
99
209 + 35 + 35 70 Abiotrophia defectiva 99
Acidobacteria sp. 94
Afipia genosp. 12 97
Sphingomonas sp. 98
Pseudomonas stutzeri 100

*qPCR, quantitative PCR; Ct, cycle threshold.

Main Article

Top of Page

USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO