Volume 16, Number 2—February 2010
Research
Tropheryma whipplei in Patients with Pneumonia
Sabri Bousbia, Laurent Papazian, Jean-Pierre Auffray, Florence Fenollar, Claude Martin, Wenjun Li, Laurent Chiche, Bernard La Scola, and Didier Raoult
Table 1
PCR test results of bronchoalveolar lavage specimens that were positive for Tropheryma whipplei DNA, collected from 6 intensive care unit patients in Marseille, France, February 2007–January 2008*
| Specimen no. |
16S rDNA
for T. whipplei |
qPCR probe |
Other bacteria identified (16S rDNA primer set) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Twhi3 |
Ct |
Twhi2 |
Ct |
Load (copy) |
Bacterium |
Identity, % |
|||
| 5 | + | + | 20 | + | 21 | 5.105 | – | 99 | |
| 10 | – | + | 30 | + | 31 | 4,8.102 | Streptococcus pneumoniae | 99 | |
| Actinobacillus pleuropneumoniae | 99 | ||||||||
| Peptostreptococcus sp. |
99 |
||||||||
| 82 | – | + | 29 | + | 28 | 5,3.103 | Streptococcus genomosp. C4 | 99 | |
| Uncultured | 99 | ||||||||
| Streptococcus sp. clone 2.17 | 89 | ||||||||
| Streptococcus sanguinis | 99 | ||||||||
| Gemella sanguinis | 99 | ||||||||
| Leptotrichia sp. | 95 | ||||||||
| Haemophilus quentini | 99 | ||||||||
| Uncultured Haemophilus sp. | 99 | ||||||||
| Peptostreptococcus sp. | 99 | ||||||||
| Granulicatella para-adiacens | 95 | ||||||||
| Prevotella sp. | 99 | ||||||||
| Prevotella melaninogenica | 99 | ||||||||
| Cloacibacterium normanense | 93 | ||||||||
| Ralstonia solanacearum | 99 | ||||||||
| Haemophilus haemolyticus | 99 | ||||||||
| Uncultured Arcobacter sp. clone DS126 | 99 | ||||||||
| Prevotella salivae | 99 | ||||||||
| Streptococcus parasanguis |
99 |
||||||||
| 86 | – | + | 31 | + | 29 | 7,6.102 | Uncultured Porphyromonas sp. clone 302E06 | 99 | |
| Uncultured Capnocytophaga sp. | 95 | ||||||||
| Gemella sanguinis | 99 | ||||||||
| Streptococcus constellatus | 99 | ||||||||
| Prevotella melaninogenica | 91 | ||||||||
| Uncultured Porphyromonas sp. | 93 | ||||||||
| Haemophilus parainfluenzae | 99 | ||||||||
| Uncultured Tannerella sp. | 98 | ||||||||
| Prevotella melaninogenica | 99 | ||||||||
| Granulicatella para-adiacens |
99 |
||||||||
| 183 | – | + | 36 | + | 37 | 50 | Staphylococcus epidermidis | 99 | |
| Porphyromonas gingivalis | 99 | ||||||||
| Pseudomonas sp. | 92 | ||||||||
| Eubacterium sp. | 94 | ||||||||
| Streptococcus anginosus | 99 | ||||||||
| Uncultured Neisseria sp. clone 502G08 | 99 | ||||||||
| Treponema sp. | 97 | ||||||||
| Clostridium sp. | 97 | ||||||||
| Acidovorax sp./Diaphorobacter sp. | 99 | ||||||||
| Eubacterium brachy | 99 | ||||||||
| Gemella haemolysans | 99 | ||||||||
| Mycoplasma orale | 95 | ||||||||
| Comamonas denitrificans | 95 | ||||||||
| Prevotella tannerae | 98 | ||||||||
| Peptostreptococcus micros |
99 |
||||||||
| 209 | – | + | 35 | + | 35 | 70 | Abiotrophia defectiva | 99 | |
| Acidobacteria sp. | 94 | ||||||||
| Afipia genosp. 12 | 97 | ||||||||
| Sphingomonas sp. | 98 | ||||||||
| Pseudomonas stutzeri | 100 | ||||||||
*qPCR, quantitative PCR; Ct, cycle threshold.
