Seropositivity for Enterocytozoon bieneusi, Czech Republic
Bohumil Sak, Zuzana Kučerová, Martin Kváč , Dana Květoňová, Michael Rost, and Evan W. Secor
Author affiliations: Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic (B. Sak, M. Kváč, D. Květoňová); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (Z. Kučerová, E.W. Secor); University of South Bohemia in České Budějovice, České Budějovice (M. Kváč, M. Rost)
Figure. Western blot analysis of serum reactivity to Enterocytozoon bieneusi proteins, Czech Republic. Serum selection: HIV-positive persons (indirect fluorescence antibody [IFA] assay titers >400); blood donors, professionals with risk exposure (IFA titers >200). Serum samples diluted 1:100. Molecular weight markers (Precision Plus Protein Standard, Bio-Rad Laboratories, Hercules, CA, USA): lane 1, positive control (HIV/AIDS patient with proved E. bieneusi infection); lane 2, negative control (seronegative blood donor); lanes 3–8, selected samples from HIV-positive persons (3–6 IFA positive); lanes 9–14, selected samples from blood donors (9–11 IFA positive); lanes 15–20, selected samples from persons with occupational exposure to animals (15–18 IFA positive). Arrow indicates the 32-KDa protein fraction. Values on the left are in kilodaltons.
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