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Volume 16, Number 4—April 2010

Dispatch

Novel Corynebacterium diphtheriae in Domestic Cats

Aron J. HallComments to Author , Pamela K. Cassiday, Kathryn A. Bernard, Frances Bolt, Arnold G. Steigerwalt, Danae Bixler, Lucia C. Pawloski, Anne M. Whitney, Masaaki Iwaki, Adam Baldwin, Christopher G. Dowson, Takako Komiya, Motohide Takahashi, Hans P. Hinrikson, and Maria L. Tondella
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (A.J. Hall, P.K. Cassiday, A.G. Steigerwalt, L.C. Pawloski, A.M. Whitney, H.P. Henrikson, M.L. Tondella); West Virginia Department of Health and Human Resources, Charleston, West Virginia, USA (A.J. Hall, D. Bixler); Public Health Agency of Canada, Winnipeg, Manitoba, Canada (K.A. Bernard); University of Warwick, Coventry, UK (F. Bolt, A. Baldwin, C.G. Dowson); National Institute of Infectious Diseases, Tokyo, Japan (M. Iwaki, T. Komiya, M. Takahashi)

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Table

Feline Corynebacterium diphtheriae isolates and reference strains used for comparison, West Virginia, 2008*

Strain Culture collection Source Diphtheria toxin GenBank accession no.
16S rRNA rpoB tox gene
CD443 ATCC BAA-1774 Cat 1, right ear Nontoxigenic FJ409572 FJ415317 FJ376656
CD448 ND Cat 1, right ear Nontoxigenic FJ409573 ND FJ422272
CD449 ND Cat 1, left ear Nontoxigenic FJ409574 ND FJ422273
CD450 ND Cat 2, left ear Nontoxigenic FJ409575 FJ415318 FJ422274
C. diphtheriae biotype mitis NCTC 10356† Human nose Nontoxigenic GQ118340 GQ409648 ND
C. diphtheriae biotype gravis NCTC 10648 Unknown Toxigenic ND ND ND
C. diphtheriae biotype gravis NCTC 11397T
ATCC 27010T Unknown Nontoxigenic GQ118341 GQ409649 ND
C. diphtheriae biotype gravis NCTC 13129
ATCC 700971 Human throat Unknown GQ118344 GQ409650 ND
C. pseudotuberculosis NCTC 3450T Sheep gland Unknown GQ118342 GQ409651 ND
C. ulcerans NCTC 12077 Human throat Unknown GQ118343 ND ND
C. ulcerans NCTC 7910 Human throat Unknown GQ118345 ND ND

*CD, Centers for Disease Control and Prevention identifier number; ATCC, American Type Culture Collection; ND, not deposited in this study; NCTC, National Collection of Type Cultures, London, UK. Additional strains used as controls for specific assays: toxigenic C. diphtheriae biotype belfanti isolates used for real-time PCR of tox gene were 718, G4182, C59, C60, C75, C76, C77; toxigenic C. diphtheriae ATCC 27012 used as positive control for Elek; C. diphtheriae NCTC 10481 and C. ulcerans CD199 used as positive and negative controls for Vero cell assay.
†NCTC 10356 is described in the NCTC catalogue as C. diphtheriae biotype mitis; however, analyses in this study found this strain to be nitrate negative and therefore consistent with C. diphtheriae biotype belfanti. Thus, it was used in this study as a belfanti reference strain.

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