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Volume 17, Number 10—October 2011
Letter

Novel Hepatitis E Virus Genotype in Norway Rats, Germany

Wen Zhang, Quan Shen, Xiuguo Hua, and Li CuiComments to Author 
Author affiliations: Jiangsu University, Jiangsu, People’s Republic of China (W. Zhang); The Ohio State University, Wooster, Ohio, USA (Q. Shen); Shanghai JiaoTong University, Shanghai, People’s Republic of China (X. Hua, L. Cui)

Main Article

Table

Primers used in study (1) of amplification of complete genome sequence of rat HEV from rat sample 63, Germany*

Designation Binding position Sequence† (5′ → 3′)
rHEV-RACEn‡ 307–284 GTGCTCATTAATAGATCGAGGGTG
rHEV-RACE‡ 336–313 GGAAGAAAACATCTGTGAATGACA
HEV-100s 78–102 CGGCCAATTCCGCCYTGGSRAATGC
rHEV-900as 905–881 TATGCCCGCCCGCACTAAACAAACT
HEV-800s 776–801 GTGCGGGCCATTGGCTGYCAYTTTGT
rHEV3300as 3099–3074 AGCCGCCATTCTGTTGGCTCCAGATT
rat5-s 2921–2943 CGCCGGGTTGTGATKGAYGAGGC
rHEV-td1-as 4062–4037 GAAATGCCCTGCCCGACCTTGCCATG
HEV-cs 3977–3999 TCGCGCATCACMTTYTTCCARAA
HEV-cas 4446–4424 GCCATGTTCCAGACDGTRTTCCA
Rat HEV-inv-s 4301–4322 GGGGCRCCYGAGTGGATGTGGA
63-5400as 5449–5428 CTCAGTCGCCATGATATGCGTA
#8-ORF2-s 5399–5421 CCCTTACTGCCTYTKCAGGAYGG
#8-ORF2-as 5604–5582 GTGGAAGTGATGGAATTCATRTC
63-5500s§ 5555–5576 CAATCCACAACAGTCCCCACGT

*HEV, hepatitis E virus; RACE, rapid amplification of cDNA ends; ORF, open reading frame.
†D = A + G + T; K = G + T ; M = A + C; R = A + G; S = G + C; Y = C + T.
‡Primers were used for 5′-RACE.
§Primer was used for 3′-RACE.

Main Article

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