Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 17, Number 10—October 2011
Dispatch

Novel Arenavirus, Zambia

Akihiro IshiiComments to Author , Yuka Thomas, Ladslav Moonga, Ichiro Nakamura, Aiko Ohnuma, Bernard M. Hang’ombe, Ayato Takada, Aaron S. Mweene, and Hirofumi Sawa
Author affiliations: Hokkaido University, Sapporo, Japan (A. Ishii, Y. Thomas, I. Nakamura, A. Ohnuma, A. Takada, H. Sawa); University of Zambia, Lusaka, Zambia (A. Ishii, Y. Thomas, L. Moonga, I. Nakamura, B. Hang’ombe, A. Takada, A. Mweene, H. Sawa)

Main Article

Figure 2

Detection of increasing viral RNA by 1-step reverse transcription PCR in a novel arenavirus, Zambia, 2009. Viral RNA was extracted from 100 μL of culture supernatant on the indicated days (top) and eluted in 20 μL of distilled water. The RNA sample was subjected to 1-step reverse transcription PCR with the specific primers 5′-TGAGAGACATTGCTTCACAATTGACATCC-3′ and 5′-TGACCCATTCTTGATGTATTGTGACTCC-3′, which were designed to amplify a 1,000-bp fragment within the determined large gene segment of Luna

Figure 2. Detection of increasing viral RNA by 1-step reverse transcription PCR in a novel arenavirus, Zambia, 2009. Viral RNA was extracted from 100 μL of culture supernatant on the indicated days (top) and eluted in 20 μL of distilled water. The RNA sample was subjected to 1-step reverse transcription PCR with the specific primers 5′-TGAGAGACATTGCTTCACAATTGACATCC-3′ and 5′-TGACCCATTCTTGATGTATTGTGACTCC-3′, which were designed to amplify a 1,000-bp fragment within the determined large gene segment of Luna virus. DNA size markers are shown in the far left lane; sizes in kb are indicated at left.

Main Article

Page created: September 23, 2011
Page updated: September 23, 2011
Page reviewed: September 23, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external