Figure 2. Detection of increasing viral RNA by 1-step reverse transcription PCR in a novel arenavirus, Zambia, 2009. Viral RNA was extracted from 100 μL of culture supernatant on the indicated days (top) and eluted in 20 μL of distilled water. The RNA sample was subjected to 1-step reverse transcription PCR with the specific primers 5′-TGAGAGACATTGCTTCACAATTGACATCC-3′ and 5′-TGACCCATTCTTGATGTATTGTGACTCC-3′, which were designed to amplify a 1,000-bp fragment within the determined large gene segment of Luna virus. DNA size markers are shown in the far left lane; sizes in kb are indicated at left.