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Volume 17, Number 5—May 2011

Research

Experimental Oral Transmission of Atypical Scrapie to Sheep

Marion M. SimmonsComments to Author , S. Jo Moore1, Timm Konold, Lisa Thurston, Linda A. Terry, Leigh Thorne, Richard Lockey, Chris Vickery, Stephen A.C. Hawkins, Melanie J. Chaplin, and John Spiropoulos
Author affiliations: Author affiliation: Veterinary Laboratories Agency–Weybridge, Addlestone, UK

Main Article

Appendix

Table. Immunohistochemical/ELISA results for sheep infected with scrapie for each tissue*

Genotype
ARR/ARR
AHQ/AHQ
Months postinoculation
12
24
12
24
Donor inoculum
ARRa
ARRa
ARRa
ARRa
ARRa
ARRb
AHQ
AHQ
AHQ
AHQ
AHQ
AHQ
Animal no. 1 2 3 4 5 6 7 8 9 10 11 12
Clinical status Norm Norm Inc Norm Norm Inc Norm Norm Norm Inc Norm Suspect
Obex –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Cerebellum† −/−† −/− −/− −/− −/− −/− −/− −/− −/− −/− −/−‡ +/+‡
Rostral medulla –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Caudal midbrain –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Rostral midbrain –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Thalamus −/− −/− −/− −/− −/− −/− −/− −/− −/− −/− +/−‡§ +/+
Occipital cortex –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Parietal cortex –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Frontal cortex −/− −/− −/− −/− −/− −/− −/− −/− −/− −/− +/+‡ +/+
Spinal cord C5 –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Spinal cord T1 –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Spinal cord T6 –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Spinal cord T12 −/− −/− –/ITT −/− −/− −/− −/− −/− −/− −/− −/− −/−
Spinal cord L5–6 –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Palatine tonsil –/ITT –/ITT –/ITT –/ITT –/ITT –/ITT –/ITT –/ITT –/ITT –/ITT –/ITT –/ITT
Spleen† −/− −/− −/− −/− −/− −/− −/− −/− −/− −/− −/− −/−
Mesenteric lymph node −/− −/− −/− −/− −/− −/− −/− −/− −/− −/− −/− −/−
Distal ileum† −/− −/− −/− −/− −/− −/− −/− −/−‡ −/−‡ −/− −/− −/−‡
RAMALT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT
Coeliaco-mesenteric plexus –/NT –/NT –/NT –/NT –/NT –/NT –/NT –/NT ITT/NT –/NT –/NT –/NT
Extraocular muscles –/ITT –/ITT –/ITT −/− –/ITT −/− –/ITT −/− −/− −/− −/− −/−
Medial retropharyngeal lymph node NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– −/− −/−
Submandibular lymph node NT/ITT NT/ITT NT/ITT NT/ITT NT/– NT/– NT/– NT/ITT NT/ITT NT/– /ITT −/−
Submandibular salivary gland NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– −/− −/−
Cervical thymus NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– −/− −/−
Prescapular lymph node NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– −/− −/−
Kidney NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– −/− −/−
Liver NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– NT/– −/− −/−
Buffy coat NT/– NT/– NT/– NT/ITT NT/ITT NT/ITT NT/– NT/– NT/– NT/ITT NT/ITT NT/ITT

*ARR/ARR, Prp genotype of 6 Poll Dorset lambs; AHQ/AHQ, PrP genotype of 6 Cheviot lambs; mpi, months post inoculation; Norm, normal; Inc, inconclusive; Suspect, suspected; NT, not tested; ITT, insufficient tissue to test; RAMALT, recto-anal mucosa–associated
lymphoid tissue; PrPSc or protease-resistant form present (+) or absent (–).
†Tissues being investigated by bioassay in Tg338 mice.
‡Tissues are positive in bioassay.
§High negative result in ELISA.

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1Current affiliation: Murdoch University School of Veterinary and Biomedical Sciences, Murdoch, Western Australia, Australia.

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