Kyasanur Forest Disease Virus Alkhurma Subtype in Ticks, Najran Province, Saudi Arabia
Figure. Phylogenetic analysis of Alkhurma viruses isolated from Ornithodoros savignyi and Hyalomma dromedarii ticks in Najran Province, Kingdom of Saudi Arabia. A 390-bp region of the core protein C and preM genes was amplified and sequenced for each of the isolates (HQ443410–6) by using primers ALK244S (5′-GTGTTGATGCGCATGATGGG-3′) and ALK665R (5′-TGCAGAAACAGTCCACATCA-3′). A maximum-likelihood analysis was conducted with available sequences in GenBank for ALK (NC_004355; 3) by using Kyasanur Forest disease (AY323490) as the outgroup and the default settings in GARLI version 0.96b8 (www.phylo.org/pdf_docs/zwicklDissertation.pdf). Scale bar indicates substitutions per site.