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Volume 18, Number 11—November 2012

Research

Antigenic Diversity of Enteroviruses Associated with Nonpolio Acute Flaccid Paralysis, India, 2007–2009

C. Durga RaoComments to Author , Prasanna Yergolkar1, and K. Subbanna Shankarappa12
Author affiliations: Author affiliations: Indian Institute of Science, Bangalore, India (C.D. Rao, K.S. Shankarappa); National Institute of Virology–World Health Organization–South East Asia Region Polio Laboratory Network, Victoria Hospital, Bangalore (P. Yergolkar); National Institute of Virology, Bangalore (P. Yergolkar); Victoria Hospital, Bangalore (P. Yergolkar)

Main Article

Figure 3

Phylogenetic analyses of viral protein 1 sequences of enterovirus 71 and echovirus 13 strains with those of reference strains representing different genogroups and subgenogroups within a serotype, India, 2007–2009. Multiple sequence alignments were performed by using ClustalW program (www.genome.jp/tools/clustalw/) and phylogenetic analysis by MEGA5 program (12) with pairwise comparison and maximum composite likelihood nucleotide substitution model. Phylogenetic trees were constructed by UPGMA (

Figure 3. . . . Phylogenetic analyses of viral protein 1 sequences of enterovirus 71 and echovirus 13 strains with those of reference strains representing different genogroups and subgenogroups within a serotype, India, 2007–2009. Multiple sequence alignments were performed by using ClustalW program (www.genome.jp/tools/clustalw/) and phylogenetic analysis by MEGA5 program (13) with pairwise comparison and maximum composite likelihood nucleotide substitution model. Phylogenetic trees were constructed by UPGMA (unweighted pair group method using arithmetic averages) with statistical significance of the phylogenetic analyses estimated by bootstrap analysis with 1,000 pseudoreplicate datasets. A and B represent phylogenetic trees of viral protein 1 sequences of enterovirus 71 and echovirus 13, respectively. The serotype, state and year of isolation of each strain and GenBank accession numbers of reference strains used are indicated. 1000B is an echovirus 1 strain. Scale bars indicate nucleotide substitutions per site.

Main Article

1These authors contributed equally to this article.

2Current affiliation: University of Horticultural Sciences, Bagalkot, India.

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