Figure 2. Sequence quality and number of ambiguous bases for 673 unidentified bacterial isolates. The median sequence length was 480 bases, with 84% of sequences in the range of 461 to 500 bases (A). The median phred sequence quality score was 45 (B). Most sequences had no ambiguous positions (n = 416, 61.8%). Up to 18 ambiguous positions were seen in isolates with multiple, nonidentical copies of the 16S rRNA gene (C). The x-axes indicate relative frequency. Numbers above columns represent isolate counts.
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