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Volume 18, Number 3—March 2012

Research

A Systematic Approach for Discovering Novel, Clinically Relevant Bacteria

Robert Schlaberg1Comments to Author , Keith E. Simmon1, and Mark A. Fisher
Author affiliations: University of Utah School of Medicine, Salt Lake City, Utah, USA (R. Schlaberg, M.A. Fisher); ARUP Laboratories, Salt Lake City (R. Schlaberg, K.E. Simmon, M.A. Fisher)

Main Article

Figure 2

Sequence quality and number of ambiguous bases for 673 unidentified bacterial isolates. The median sequence length was 480 bases, with 84% of sequences in the range of 461 to 500 bases (A). The median phred sequence quality score was 45 (B). Most sequences had no ambiguous positions (n = 416, 61.8%). Up to 18 ambiguous positions were seen in isolates with multiple, nonidentical copies of the 16S rRNA gene (C). The y-axis indicates relative frequency in percent. Numbers above columns represent is

Figure 2. Sequence quality and number of ambiguous bases for 673 unidentified bacterial isolates. The median sequence length was 480 bases, with 84% of sequences in the range of 461 to 500 bases (A). The median phred sequence quality score was 45 (B). Most sequences had no ambiguous positions (n = 416, 61.8%). Up to 18 ambiguous positions were seen in isolates with multiple, nonidentical copies of the 16S rRNA gene (C). The x-axes indicate relative frequency. Numbers above columns represent isolate counts.

Main Article

1These authors contributed equally to this article.

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