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Volume 18, Number 4—April 2012

Dispatch

Detection of Plasmodium spp. in Human Feces

Milan Jirků1, Kateřina Pomajbíková1, Klára J. Petrželková, Zuzana Hůzová, David Modrý, and Julius LukešComments to Author 
Author affiliations: Author affiliations: Biology Centre, Institute of Parasitology, ASCR, České Budějovice, Czech Republic (M. Jirků, D. Modrý, J. Lukeš); Faculty of Science, University of South Bohemia, České Budějovice (M. Jirků, J. Lukeš); University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic (K. Pomajbíková, D. Modrý); CEITEC, Brno (D. Modrý); Institute of Vertebrate Biology, ASCR, Brno, and Zoo Liberec, Liberec, Czech Republic (K.J. Petrželková); Health Institute, Prague, Czech Republic (Z. Hůzová)

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Figure

Top, agarose gel electrophoresis of nested PCR products of human fecal samples amplified with primers (pairs DW2-F + DW2-R and CYTB1-F + CYTB2-R) and stained with ethidium bromide. Bottom, autoradiograph of a Southern blot of the same gel. α-32P-ATP–labeled acytochrome B gene of Plasmodium falciparum was used as a probe. Lanes 1–6, samples from humans with malaria (the infection sample in lane 6 is weak); lane 2, spurious amplicon; lane 7, sample from an uninfected person; lane M, 1-kb molecular

Figure. Top, agarose gel electrophoresis of nested PCR products of human fecal samples amplified with primers (pairs DW2-F + DW2-R and CYTB1-F + CYTB2-R) and stained with ethidium bromide. Bottom, autoradiograph of a Southern blot of the same gel. α-32P-ATP–labeled acytochrome B gene of Plasmodium falciparum was used as a probe. Lanes 1–6, samples from humans with malaria (the infection sample in lane 6 is weak); lane 2, spurious amplicon; lane 7, sample from an uninfected person; lane M, 1-kb molecular mass (Invitrogen, Carlsbad, CA, USA).

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1These authors contributed equally to this article.

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