Emerging Infectious Disease ISSN: 1080-6059

Detection of Plasmodium spp. in Human Feces

Milan Jirků¹, Kateřina Pomajbíková¹, Klára J. Petrželková, Zuzana Hůzová, David Modrý, and Julius Lukeš

Author affiliations: Author affiliations: Biology Centre, Institute of Parasitology, ASCR, České Budějovice, Czech Republic (M. Jirků, D. Modrý, J. Lukeš); Faculty of Science, University of South Bohemia, České Budějovice (M. Jirků, J. Lukeš); University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic (K. Pomajbíková, D. Modrý); CEITEC, Brno (D. Modrý); Institute of Vertebrate Biology, ASCR, Brno, and Zoo Liberec, Liberec, Czech Republic (K.J. Petrželková); Health Institute, Prague, Czech Republic (Z. Hůzová)

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Figure

Top, agarose gel electrophoresis of nested PCR products of human fecal samples amplified with primers (pairs DW2-F + DW2-R and CYTB1-F + CYTB2-R) and stained with ethidium bromide. Bottom, autoradiograph of a Southern blot of the same gel. α-32P-ATP–labeled acytochrome B gene of Plasmodium falciparum was used as a probe. Lanes 1–6, samples from humans with malaria (the infection sample in lane 6 is weak); lane 2, spurious amplicon; lane 7, sample from an uninfected person; lane M, 1-kb molecular

Figure. Top, agarose gel electrophoresis of nested PCR products of human fecal samples amplified with primers (pairs DW2-F + DW2-R and CYTB1-F + CYTB2-R) and stained with ethidium bromide. Bottom, autoradiograph of a Southern blot of the same gel. α-³²P-ATP–labeled acytochrome B gene of Plasmodium falciparum was used as a probe. Lanes 1–6, samples from humans with malaria (the infection sample in lane 6 is weak); lane 2, spurious amplicon; lane 7, sample from an uninfected person; lane M, 1-kb molecular mass (Invitrogen, Carlsbad, CA, USA).

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¹These authors contributed equally to this article.

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