Volume 18, Number 4—April 2012
Letter
Serologic Evidence of Orthopoxvirus Infection in Buffaloes, Brazil
Felipe Lopes de Assis1, Graziele Pereira1, Cairo Oliveira, Gisele Olinto Libânio Rodrigues, Marcela Menezas Gomes Cotta, Andre Tavares Silva-Fernandes, Paulo Cesar Peregrino Ferreira, Cláudio Antônio Bonjardim, Giliane de Souza Trindade, Erna Geessien Kroon, and Jônatas Santos Abrahão
Table
Results of testing for orthopoxvirus seropositivity in milk buffalo herds, Minas Gerais State, Brazil, October 2010*
| Test | No. (%) samples |
|---|---|
| PRNT | |
| Total positive | 15 (31.2) |
| Titer | |
| 20 | 6 (40.0) |
| 40 | 5 (33.3) |
| 80 | 2 (13.3) |
| 160 | 2 (13.3) |
| Total negative | 33 (68.7) |
| ELISA | |
| Total positive | 17 (35.4) |
| Total negative | 31 (64.6) |
| PRNT and ELISA positive | 14 (29.2) |
*Serum samples were collected from 48 female buffaloes used for milk production. A positive titer was defined as the highest dilution that inhibited >70% of viral plaques relative to the level of inhibition of the negative controls. Samples also underwent ELISA for orthopoxvirus IgG as described (4). PRNT, plaque-reduction neutralization test.
1These authors contributed equally to this article.
