Volume 18, Number 4—April 2012
Serologic Evidence of Orthopoxvirus Infection in Buffaloes, Brazil
|Test||No. (%) samples|
|Total positive||15 (31.2)|
|Total negative||33 (68.7)|
|Total positive||17 (35.4)|
|Total negative||31 (64.6)|
|PRNT and ELISA positive||14 (29.2)|
*Serum samples were collected from 48 female buffaloes used for milk production. A positive titer was defined as the highest dilution that inhibited >70% of viral plaques relative to the level of inhibition of the negative controls. Samples also underwent ELISA for orthopoxvirus IgG as described (4). PRNT, plaque-reduction neutralization test.
1These authors contributed equally to this article.